玉米自交系郑58和丹340幼胚再生体系的建立  

Establishment of Plant Regeneration System from Immature Embryos of Maize Inbred Lines Zheng58 and Dan340

作　　者：邓川[1,2] 牛亚玲[3] 杨振明[1,2] 杨德光[4] 边鸣镝[1,2] 

机构地区：[1]吉林大学农学部/土壤-植物分子遗传学实验室,长春130062 [2]吉林大学农学部植物科学学院,长春130062 [3]白城师范学院生物系,吉林白城137000 [4]东北农业大学农学院,哈尔滨150030

出　　处：《玉米科学》2011年第6期20-24,共5页Journal of Maize Sciences

基　　金：转基因生物新品种培育重大专项(20092X08003-009B);211工程3期重点学科建设项目

摘　　要：研究选取9个优良玉米品种的自交系,以幼胚为外植体,以N6、MS培养基为基本培养基,诱导愈伤组织,探讨基因型、培养基类型、外植体大小、激素浓度等因素对愈伤组织诱导率的影响,分析不同基因型愈伤组织生长状态的差异,对诱导成功的愈伤组织进行分化和再生。研究结果表明,基因型对愈伤组织诱导率起决定作用,且基因型和培养基之间存在相互作用;外植体大小在1.5～2.0 mm时的幼胚较适合诱导胚性愈伤组织;2,4-D对玉米幼胚愈伤组织的诱导形成起关键作用,浓度在2.0～2.5 mg/L时大多能诱导出胚性愈伤组织。Callus was induced from immature embryos of 9 maize inbred lines on MB medium and N6 medium. The effects of different genotypes, different mediums, explants sizes and different concentrations of phytohormone on the callus induction of immature embryos were studied. Differences in callus growth state from different genotypes were also analyzed. Regeneration system was successfully established via the callus pathway. The results showed that genotypes have significant effects on callus induction. Both genotype and medium affected the frequency of embryogenic callus formation. The choice size was 1.5-2.0 mm young embryos as explants to be advantageous to obtain perfect callus. 2,4-dichlorophenoxyacetic acid （2,4-D）was necessary in callus induction, and the optimal concentration for embryogenic callus formation was 2.0-2.5 mg/L.

关 键 词：玉米 幼胚 愈伤组织 诱导 再生体系 

分 类 号：S513.035.3[农业科学—作物学]