远缘背景下玉米主要性状的QTLs分析  

QTLs Analysis of the Major Characters of Inbred Lines Derived from Maize(Zea mays L.)×Teosinte(Zea mexicana Schard)

作　　者：石海春[1] 乔晓[1] 柯永培[1] 傅体华[1] 袁继超[1] 余学杰[1] 

机构地区：[1]四川农业大学农学院,四川温江611130

出　　处：《玉米科学》2011年第6期30-39,共10页Journal of Maize Sciences

基　　金：四川省教育厅青年基金项目(07ZB062);四川省教育厅重点专项(08ZZ002);四川省应用基础研究项目(2008JY0101);四川省"十一五"玉米育种攻关项目(2006-3-16)

摘　　要：通过远缘杂交导入大刍草基因,创造玉米新种质,并对相关重要性状进行QTL定位,明确其QTL数目、位置和基因效应。以玉米自交系K169×1147构建的202个F2:3家系为作图群体,构建具有132对SSR标记的玉米遗传图谱,覆盖整个基因组1 979.6 cM,平均图距15.0 cM。利用完美区间作图法进行QTL定位分析,共检测到14个主要农艺和经济性状中的68个QTLs。其中,控制株高、穗位高和叶面积的有14个QTLs,单个性状的QTL为1～7个,每个QTL的作用可解释表型变异的1.71%～12.98%;影响雄穗性状的有11个QTLs,单个性状的QTL为3～8个,每个QTL的作用可解释表型变异的3.61%～14.87%;影响穗长等经济性状的有43个QTLs,单个性状的QTL为1～7个,每个QTL可解释的表型变异为3.87%～20.09%。New maize germplasm has been obtained through transferring genes from Z. mexicana Schard by distant hybridization, as well as the QTL locations and effects on the important agronomic traits, are of significant importance to improve maize breeding, this knowledge may provide a good foundation for molecular breeding in maize. Therefore, in current study, 202 F2：3family lines derived from a single cross between the inbred line K169 and 1147 were used as the mapping population. A maize genetic linkage map with 132 pairs of SSR markers was established, which covered 1979.6 cM of the whole genome with the average map distance of 15.0 cM. A total of 68 QTLs were identified in 14 major agronomy and yield traits, of which 14 QTLs contributed to plant height, ear height and leaf area, and 1-7 QTLs for single character were detected, with each QTL accounting for phenotypic variation from 1.71% to 12. 98%; there were 11 QTLs influencing the branch traits with 3-8 QTLs of single character, and the effect of each QTL could explain 3.61% to 14. 87% of phenotypic variation. Moreover, 43 QTLs influenced the yield and other economic characters with 1-7 QTLs of a single character to explain 3.87 % to 20.09 % of phenotypic variation.

关 键 词：玉米 大刍草 远缘杂交 主要性状 QTL定位 

分 类 号：S513.035[农业科学—作物学]