不同化性亚洲玉米螟遗传多样性的AFLP分析  

Analysis on the Genetic Diversity of Different Voltinism Types Asian Corn Borer [Ostrinia furnacalis(Guenée)] by AFLP Markers

作　　者：周淑香[1] 鲁新[1] 刘宏伟[1] 丁岩[1] 张国红[1] 李丽娟[1] 王瑞荣[2] 

机构地区：[1]吉林省农业科学院植物保护研究所,吉林公主岭136100 [2]吉林省桦甸市永吉街农业站,吉林桦甸132400

出　　处：《玉米科学》2011年第6期108-111,共4页Journal of Maize Sciences

基　　金：吉林省科技发展计划重大项目(20096025;20096029);吉林省农业科学院博士启动基金

摘　　要：从50对AFLP引物中筛选出9对多态性高、扩增稳定、重复性好的引物,对敦化一化型、洮南二化型和公主岭二化型3个地理种群共24个个体进行遗传多样性分析,研究不同化性亚洲玉米螟间的遗传关系。结果表明,9对引物共扩增出1 127条清晰可辨的DNA条带,其中多态性条带1 029条,平均多态性比例为91.30%;亚洲玉米螟不同种群个体间遗传相似性指数分布在0.430 7～0.667 5之间;同一种群内个体间的遗传相似性指数敦化一化型为0.439 9～0.524 0,洮南二化型为0.487 8～0.667 5,公主岭二化型为0.459 7～0.563 9,遗传相似性较低,表明亚洲玉米螟遗传多样性十分丰富。聚类分析结果表明,供试的24个亚洲玉米螟聚成两大类,敦化一化型聚为一类,洮南二化型和公主岭二化型聚为一类。For further understanding the population dynamics and improving the forecast and control of this species, the DNA polymorphism of 3 geographic populations and different voltinism types was analyzed by using amplified fragment length polymorphism （AFLP） technique. The results showed that 1 127 bands were amplified with 9 pairs of primers and 1 029 polymorphic loci were found, which accounted for 91.30%. Genetic similarity coefficients of Asian corn borer individuals were changed from 0.430 7 to 0.667 5. By cluster analysis, twenty four accessions were grouped into two groups, Dunhua univoltine population is clustered in one group, Gongzhuling bivoltine and Taonan bivoltine populations are clustered in another group.

关 键 词：亚洲玉米螟 AFLP 遗传多样性 聚类分析 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]