艾灸对脾虚大鼠小肠上皮线粒体超微结构及呼吸链酶含量的影响  

Effect of moxibustion on mitochondrial ultrastructure and contents of mitochondrial enzymes in the intestinal epithelium of spleen-deficiency rats

作　　者：易受乡[1] 彭艳[1] 彭芬[1] 常小荣[1] 龙奕文[1] 林亚平[1] 

机构地区：[1]湖南中医药大学针灸推拿学院经穴与脏腑相关重点研究室国家中医药管理局三级实验室,湖南省长沙市410007

出　　处：《世界华人消化杂志》2011年第29期3028-3034,共7页World Chinese Journal of Digestology

基　　金：国家重大基础研究(973)基金资助项目;No.2009CB522904;湖南省教育厅基金资助项目;No.11C0965~~

摘　　要：目的:观察艾灸足三里等穴对脾虚大鼠一般症状积分、空肠组织线粒体结构、琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)含量及其亚基蛋白COXⅠ表达的影响,探讨艾灸温补脾胃改善脾虚证的作用机制.方法:40只SD大鼠随机分为4组:A空白对照组,B脾虚模型组,C艾灸穴位组(脾虚+艾条温和灸组),D中药对照组(脾虚+四君子汤组),每组10只.200%大黄水浸剂灌胃造成脾虚大鼠模型,艾灸足三里、中脘、关元、脾俞、胃俞等穴,观察大鼠一般症状,透射电镜观察大鼠空肠组织线粒体结构,酶联免疫法(ELISA)测定空肠上皮SDH和CCO含量,免疫印记(Western blot)方法测定空肠上皮细胞内细胞色素氧化酶亚基Ⅰ(Cytochrome oxidaseⅠ,COXⅠ)蛋白表达.结果:与A组比较,B组脾虚模型大鼠脾虚症状积分显著增加(P<0.01),空肠上皮细胞线粒体超微结构严重破坏,空肠上皮细胞SDH和CCO含量及COXⅠ表达较A组显著降低(P<0.05或0.01).经艾灸足三里等穴或四君子药液灌胃治疗的C、D组空肠上皮细胞线粒体超微结构轻微损伤,空肠上皮细胞SDH、CCO含量及COXⅠ表达量均显著高于B组(0.387±0.076,0.343±0.053vs0.277±0.037;0.246±0.058,0.231±0.038vs0.167±0.021;0.941±0.204,1.040±0.214vs0.481±0.131,P<0.05或0.01),C、D两组比较无显著性差异.结论:艾灸足三里等穴可温补脾胃,改善大鼠脾虚症状,其机制与促进脾虚大鼠小肠上皮细胞线粒体结构恢复,增加呼吸链酶含量及其亚基蛋白的表达有关,提示艾灸可通过促进线粒体结构和功能的恢复,改善能量代谢.AIM： To observe the effect of moxibustion on symptom scores, mitochondrial ultrastructure, contents of succinodehydrogenase （SDH） and cytochrome oxidase （CCO）, expression of COX I in the jejunal epithelium of spleen-deficiency rats and to explore the mechanism by which moxibustion reinforces the spleen-stomach. METHODS： Forty healthy Sprague-Dawley rats were randomly assigned into four groups： A （blank group; normal rats）, B （model group; spleen-deficiency rats）, C （moxibustion group; spleen-deficiency rats intragastrically given Sijunzi Decoction） and D （herbs group; spleen-deficiency rats receiving moxibustion at Zusanli, Zhongwan, Guanyuan, Pishu and Weishu points）. An animal model of spleen-deficiency was established by intragastric administration of Chinese Rhubarb Infusion. Common symptoms were recorded. Mitochondrial ultrastructure of the jejunal epithelium was observed by electron microscopy. Enzyme-linked immunosorbent assay was used to detect the contents of SDH and CCO. The expression of COX I was detected by Western blot. RESULTS： Compared to group A, symptom score increased significantly （P 0.01）, the contents of SDH and CCO and expression of COX I decreased significantly （P 0.05 or 0.01）, and the mitochondrial ultrastructure of the jejunal epithelium was terribly destroyed in group B. Compared to group B, the mitochondrial ultrastructure of the jejunal epithelium was only mildly destroyed, and the contents of SDH and CCO and expression of COX I increased significantly （0.387 ± 0.076, 0.343 ± 0.053 vs 0.277 ± 0.037; 0.246 ± 0.058, 0.231 ± 0.038 vs 0.167 ± 0.021; 0.941 ± 0.204, 1.040 ± 0.214 vs 0.481 ± 0.131, P 0.05 or 0.01） in groups C and D. There were no significant differences in the above parameters between groups C and D. CONCLUSION： Moxibustion could warm and reinforce the spleen-stomach and relieve symptoms of spleen-deficiency possibly by promoting the recovery of mitochondrial ultrastructure of the intestinal epithelium and increasing th

关 键 词：艾灸 足三里 脾虚 线粒体 四君子汤 

分 类 号：R245[医药卫生—针灸推拿学]