阿维菌素对家蚕血细胞DNA变异的影响  

Effects of Avermectin on DNA Variation in Hemocytes of Silkworm

作　　者：沈卫锋[1,2] 孟智启[2] 王强[3] 牛宝龙[2] 何丽华[2] 刘岩[2] 翁宏飚[2] 陈玉银[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江省农业科学院蚕桑研究所,杭州310021 [3]浙江省农业科学院农产品质量标准研究所,杭州310021

出　　处：《蚕业科学》2011年第6期1025-1028,共4页ACTA SERICOLOGICA SINICA

基　　金：国家高技术研究发展计划"863"项目(No.2011AA10-0806)

摘　　要：利用RAPD标记技术检测杀虫剂阿维菌素对家蚕血细胞DNA的损伤,作为评价其对蚕体毒性的依据。分别用1、2、4、8μg/L阿维菌素药液处理的桑叶给4龄起蚕添食,96 h后对家蚕血细胞的基因组DNA进行RAPD分析。24条RAPD引物对5个样品基因组DNA扩增产生的清晰条带数为143条,其中多态性片段19条,多态性带数比率为13.29%。用Ntsyspc 2.1软件计算出不同处理样品血细胞DNA间的遗传相似系数分布在0.867～0.993,树状聚类图显示正常样品与1、2、4μg/L阿维菌素药液处理的3个样品聚为一类,而8μg/L阿维菌素药液处理的样品单独聚为一类,说明该样品血细胞DNA的变异最大。研究结果提示,RAPD标记作为杀虫剂对家蚕的毒性检测标志技术,可准确预警蚕区农药使用对养蚕生产存在的潜在危害。DNA damage of silkworm（Bombyx mori） hemocytes detected by RAPD marker technology could be used as a reference for evaluating the toxicity of avermectin to silkworm.The 4th instar newly exuviated silkworm larvae were fed with mulberry leaves treated with 1,2,4,and 8 μg/L avermectin.After 96 hours,the genomic DNAs of silkworm hemocytes were isolated for RAPD assay.By using 24 RAPD primers to amplify the genomic DNAs of five samples,143 clearly distinguishable bands were obtained,among which 19 bands were polymorphic,showing a polymorphism percentage of 13.29%.Calculation with Ntsyspc 2.1 software displayed that the genetic similarity coefficients between hemocytic DNAs from different treatments were in the range of 0.867 to 0.993.The result of dendrogram showed that the samples treated with 1,2,and 4 μg/L avermectin and the normal sample were clustered into one group,and the sample treated with 8 μg/L avermectin was in a separate group,indicating that 8 μg/L of avermectin caused the most serious effect on DNA integrality.These results showed that RAPD molecular marker technology,as a measure of detecting toxicity of pesticides to silkworm,can provide an accurate early warning to the potential danger of pesticide application to silkworm rearing in sericultural areas.

关 键 词：阿维菌素 家蚕 毒性 随机扩增多态性DNA DNA变异 

分 类 号：S881[农业科学—特种经济动物饲养]