水相合成CdTe量子点对人宫颈癌细胞(SiHa)的毒性研究  

Studies on Cytotoxicity of Aqueous CdTe Quantum Dots Using Human Cervical Cancer SiHa Cells as a Model

作　　者：祝欣[1] 董朝青[1] 王金杰[1] 黄香宜[1] 李众[1] 任吉存[1] 

机构地区：[1]上海交通大学化学化工学院金属基复合材料国家重点实验室,上海200240

出　　处：《分析科学学报》2011年第6期681-687,共7页Journal of Analytical Science

基　　金：国家自然科学基金(No.20905048);国家基础研究计划(No.2009CB930400)

摘　　要：以人宫颈癌细胞(SiHa)为模型,采用WST-1试剂法对实验室合成的水溶性CdTe量子点的细胞毒性进行了考察。研究发现,CdTe量子点毒性具有明显的尺寸效应、时间效应和剂量效应,且量子点表面状态对其毒性有较大影响。采用生物大分子变性牛血清白蛋白(dBSA)修饰量子点,其毒性与裸量子点无明显差异;经巯基聚乙二醇(thiol-PEG)修饰后量子点毒性稍有减小;在CdTe量子点表面包覆CdS和ZnS壳层后,量子点毒性显著减小。将100μg/mL CdTe/CdS/ZnS量子点与SiHa细胞共培养48h,细胞存活率仍高达89.3%,共培养24h则对细胞几乎无影响。The cytotoxicity of water-soluble CdTe guantum dots（QDs） was evaluated using human cervical cancer SiHa cells as a model using WST-1 viability assay method.Our results revealed that the cell viability significantly decreased with smaller size of QDs,higher concentration and longer exposure time.Then,surface modifications were conducted to improve the suface state of CdTe QDs.It was found that coating QDs with dBSA contributed little for reduction of their cytotoxicity,and the PEG-coated QDs showed less cytotoxicity compared to the QDs core.After the coating of CdS and ZnS shell,the cytotoxicity of QDs decreased remarkably.Little reduction of cell viability was observed after 24 hours of CdTe/CdS/ZnS QDs exposure,and cell viability even kept at 89.3% after 48 hours.

关 键 词：CDTE量子点 SIHA细胞 毒性 修饰 

分 类 号：O657[理学—分析化学]