盐酸胍诱导的猪血红蛋白分子解离过程中各亚基的分布  

Distribution of the Subunits of Porcine Hemoglobin Molecules Induced by Guanidine Hydrochloride

作　　者：段栋[1] 蔚萍[1] 边六交[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《分析科学学报》2011年第6期688-692,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21075097)

摘　　要：以内源荧光光谱、荧光相图、高效凝胶排阻色谱和相对释氧曲线等方法,研究了盐酸胍诱导的猪血红蛋白分子解离过程中各亚基的分布状况。实验发现,当溶液中盐酸胍浓度小于3.5mol/L时,猪血红蛋白分子仅仅以四聚体形式存在;而当溶液中盐酸胍浓度达到和超过3.5mol/L时,部分猪血红蛋白分子开始从四聚体解离成二聚体和单体,并且随着溶液中盐酸胍浓度的增加,单体含量逐渐增加,二聚体含量逐渐减小,四聚体含量变化不大。通过比较猪血红蛋白分子在不同浓度盐酸胍溶液中的结构变化和相对释氧曲线,发现猪血红蛋白分子的活性变化快于它们的结构变化。The distribution of the subunits in the dissociation of porcine hemoglobin molecules induced by guanidine hydrochloride was studied by using intrinsic fluorescence emission spectrum,fluorescence phase diagram,high-performance size-exclusion chromatography and relative oxygen release curve.The experimental results showed that when sguanidine hydrochloride concentration in denaturation solution was below 3.5 mol/L,porcine hemoglobin molecules existed only as a form of their native tetramers;while when guanidine hydrochloride concentration was equal to or more than 3.5 mol/L,part of them started to dissociate from their tetrameric molecules into their dimeric and monomeric molecules,and with the increasing of guanidine hydrochloride concentration in denaturation solution,the monomeric molecules continuously increased,dimeric molecules continuously decreased and tetrameric molecules did not change much.At the same time,by comparing the structural changes of porcine hemoglobin molecules with their relative oxygen release curves under different guanidine hydrochloride concentrations in denaturation solution,it was found that the biological activity changes of porcine hemoglobin molecules were faster than their structural changes during their dissociation processes.

关 键 词：猪血红蛋白分子 解离 分布 盐酸胍 

分 类 号：O657[理学—分析化学]