分光光度法测定固相表面酰肼活化基团  

Quantitative Analysis of Surface-Immobilized Hydrazide Groups by Spectrophotometry

作　　者：周雷激[1] 任志敏[1] 赵征寰[1] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005

出　　处：《分析科学学报》2011年第6期764-766,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.20775065;No.20835005);教育部高校博士点基金(No.20070384023);国家基础科学人才培养基金(No.J1030415)

摘　　要：建立了一种测定固相表面酰肼基团含量的方法。运用Fe(Ⅲ)-邻二氮菲显色体系,通过固相表面具有较强还原性的酰肼基团将Fe(Ⅲ)还原为Fe(Ⅱ),生成的Fe(Ⅱ)可与邻二氮菲生成稳定的桔红色络合物,从而利用分光光度法测定溶液体系吸光度来表征固相表面酰肼基团的含量。该方法在0～0.02μmol/mL范围内呈良好线性关系,线性相关系数r=0.9975,检出限为5.4×10-4μmol/mL。用该方法测出微珠固相表面酰肼基团的活化效率为72%。方法可以很好地应用于固相载体表面酰肼基团的测定。A measurement approach of hydrazide groups on solid-phase surface by spectrophotometry was reported.By reducing Fe（Ⅲ） to Fe（Ⅱ） in solutions with microbead surface-conjugate hydrazide groups,a stable orange-red complex of Fe（Ⅱ）-orthophenanthroline was formed.The content of hydrazide groups on the bead surface was then characterized by spectrophotometric measurement.This method has advantages of high sensitivity,simplicity and practicability.The linear range is from 0 to 0.02 μmol/mL with the linear correlation coefficient of 0.9975 and detection limit of 5.4×10-4 μmol/mL（S/N=3）.In this method,72% of the activation efficiency of the hydrazide groups activated on the carboxylated microbead surface was detected.It is promising to apply the approach to the quantitative assessment of hydrazide groups on various solid-phase surfaces.

关 键 词：微珠 酰肼 活化效率 Fe(Ⅲ)-邻二氮菲 分光光度法 

分 类 号：O657.32[理学—分析化学]