HPLC测定藏药五脉绿绒蒿中甲氧基淡黄巴豆亭碱的含量  

Determination of o-methylflavinantine in a Tibetan medicine Meconopsis quintuplinervia by HPLC

作　　者：师霞[1,2] 程芳[1,3] 郭玫[1,3] 邵晶[1,3] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000 [3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

出　　处：《中国中药杂志》2011年第23期3290-3292,共3页China Journal of Chinese Materia Medica

基　　金：甘肃省教育厅项目(0706-02)

摘　　要：目的:建立藏药五脉绿绒蒿中甲氧基淡黄巴豆亭碱的含量测定方法。方法:Hypersil-Keystone-C18色谱柱(4.6 mm×250 mm,5μm),柱温20℃,流动相乙腈-0.012%冰醋酸水溶液(50∶50),流速1.0 mL.min-1,检测波长237 nm。结果:甲氧基淡黄巴豆亭碱在0.2～2.4μg呈良好的线性关系(r=0.999 7);平均加样回收率为96.26%,RSD 1.2%。结论:本方法稳定易行、重复性好,可用于藏药五脉绿绒蒿药材的质量评价。Objective： To develop an HPLC method for the determination of a Tibetan medicine Meconopsis quintuplinervia. Method： A Hypersil-Keystone-C18 column （4.6 mm×250 mm,5 μm） was used with the isocratic elution of acetonitrile and 0.012% glacial acetic acid. The flow rate was 1.0 mL·min^-1, and the detection wavelength was set at 237 nm. Result： The linear range of O-methylflavinantine was 0.2-2.4 μg （r=0.999 7）. The average recovery was 96.26%. Conclusion： The developed method was reliable, and can be used for the quality control of M. quintuplinervia Regel.

关 键 词：藏药 五脉绿绒蒿 甲氧基淡黄巴豆亭碱 高效液相色谱法 

分 类 号：R29[医药卫生—民族医学]