天南星药材的HPLC指纹图谱研究  

Study on HPLC fingerprint chromatograms of Arisaematis Rhizoma

作　　者：罗芬[1] 陆丹[1] 池玉梅[1] 吴皓[1] 郁红礼[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中国中药杂志》2011年第23期3302-3305,共4页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI09806-10);国家自然科学基金项目(30973939)

摘　　要：目的:研究天南星药材的指纹图谱。方法:采用HPLC,以Lichrospher C18色谱柱(4.6 mm×200 mm,5μm),乙腈-水(含0.1%乙酸)为流动相,流速1.0 mL.min-1,梯度洗脱,检测波长270 nm,柱温30℃。结果:分析天南星药材色谱图的14个共有峰,以腺苷为参照物,11批药材的相似度均达到0.9,而与半夏属的虎掌南星和半夏有显著差异,并结合保留时间和紫外光谱分析,指认了腺嘌呤、次黄嘌呤、黄嘌呤、尿苷、鸟苷、腺苷、夏佛托苷、异夏佛托苷的峰位。结论:该法重复性好、专属性强,可用于天南星药材的真伪鉴别和评价。The fingerprint chromatograms of Arisaematis Rhizoma were established by HPLC. The analysis was performed on a Lichrospher C18 （4.6 mm×200 mm, 5 μm）column with acetonitrile-water （containing 0.1% acetic acid） as mobile phase at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 270 nm, and the column temperature was 30 ℃. The similarities of the fingerprint chromatograms were calculated over 0.9 between 11 batches of Arisaematis Rhizoma samples by analyzing 14 common peaks with adenosine as reference substance. However, their fingerprint chromatograms were significantly different from those of Pinellia pedatisecta and P. ternate. Adenine, hypoxanthine, xanthine, uridine, guanosine, adenosine, schaftoside, and isoschaftoside were identified by comparing the retention times and their ultraviolet spectra. The method is repeatable, exclusive and can be used for identification and evaluation of Arisaematis Rhizoma.

关 键 词：天南星 HPLC 指纹图谱 

分 类 号：R284[医药卫生—中药学]