戊己丸不同配伍对代表成分胆汁排泄的影响  

Effect of different compatibility of Wuji Wan on bile excretion of representative ingredient

作　　者：王怡薇[1] 张英丰[2] 李玉洁[1] 杨伟鹏[1] 王彦礼[1] 陈颖[1] 杨庆[1] 李涛[1] 董宇[3] 朱晓新[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]广州中医药大学,广东广州510006 [3]中国中医科学院广安门医院,北京100053

出　　处：《中国中药杂志》2011年第23期3319-3326,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(30930114);国家"重大新药创制"科技重大专项(2009ZX09301-005-009);中国中医科学院基本科研业务费自主选题项目(ZZ2006114;ZZ20090211);中医药行业科技专项(200807036)

摘　　要：目的:探讨戊己丸不同配伍时,代表成分胆汁排泄的变化。从胆汁排泄的角度解释戊己丸的配伍机制。方法:采用正交设计得到9个戊己丸复方,加上9个单味药,共18个复方。于十二指肠给药后不同时间点取胆汁,用LC-MS测定代表性成分的胆汁浓度,以此计算胆汁排泄率,药-时曲线下面积,并对结果进行方差和极差分析。结果:吴茱萸与小檗碱、巴马汀胆汁排泄呈负相关;白芍与小檗碱、巴马汀胆汁排泄呈正相关;黄连、白芍与吴茱萸碱、吴茱萸次碱胆汁排泄呈负相关;并且得到了针对各代表成分胆汁排泄的最优配伍比例。结论:吴茱萸抑制黄连代表性成分胆汁排泄;白芍促进黄连代表性成分胆汁排泄;黄连、白芍抑制吴茱萸代表性成分胆汁排泄;黄连、吴茱萸抑制白芍代表性成分的胆汁排泄。Objective： To research the representative ingredient excretion in bile using the different compatibilities of Wuji Wan, in order to indicate the regularity of compatibility. Method： L9（34） orthogonal design table was used, in addition 9 simples, altogether 18 compatibilities. After duodenal administration bile at different time spot was collected for LC-MS detection. Result： The excretion of Evodiae Fructus was negative correlated with that of berberine and palmatine in bile. The excretion of Paeoniae Radix Alba was positive correlated with that of berberine and palmatine in bile. The excretion of Coptidis Rhizoma, Paeoniae Radix Alba was negative correlated with that of evodiamine, rutaecarpine; meanwhile, the most superior proportion was also caculated which promote the representative ingredient excrete from bile. Conclusion： Evodiae fructus suppresses representative ingredients of Coptidis Rhizoma excrete through bile. Paeoniae Radix Alba promote suppresses representative ingredients of Coptidis Rhizoma excrete through bile. Coptidis Rhizoma, paeoniae Radix Alba suppresses representative ingredients of Evodiae Fructus excrete through bile. Coptidis Rhizoma, Evodiae Fructus suppresses representative ingredients of Paeoniae Radix Alba excrete through bile.

关 键 词：戊己丸 小檗碱 巴马汀 吴茱萸碱 吴茱萸次碱 芍药苷 胆汁排泄 配伍机制 

分 类 号：R285.5[医药卫生—中药学]