人端粒逆转录酶与Snapin蛋白相互作用的鉴定  

Identification of the Interaction Between the Human Telomere Reverse Transcriptase and Snapin Protein

作　　者：杨平勋[1] 白云秀[1] 黄翠芬[1] 黄君健[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2011年第6期793-795,共3页Letters in Biotechnology

摘　　要：目的:研究人端粒逆转录酶(hTERT)与Snapin蛋白的相互作用。方法:将hTERT基因和Snapin基因分别构建到pGBKT7和pGADT7载体中,用酵母双杂交方法在酵母中验证其相互作用;在293T细胞中,共转染带有Flag标签的hTERT及带有GFP标签的Snapin质粒,进行免疫共沉淀;将GST融合的Snapin纯化蛋白与hTERT进行GST-pull down,验证其相互作用。结果:3种方法都证明hTERT能够与Snapin相互作用。结论:Snapin蛋白能够与hTERT相互作用,为研究Snapin参与调控端粒酶的功能活动提供了一些线索。Objective： To identify the interaction of human telomere reverse transcriptase（hTERT） and Snapin.Methods： The hTERT gene and Snapin gene were constructed into pGBKT7 and pGADT7 vector respectively,the yeast two-hybrid technique was used to confirm their interaction in yeast cells.The flag tagged hTERT and GFP fused Sanpin vectors were contransfected into 293T cells,and immunoprecipitation and GST-pull down were performed to confirm the interaction.Results： Confirmed by the three techniques,Snapin can interact with hTERT.Conclusion： The interaction between the hTERT and Snapin which provides clues for the research of Snapin as a regulator in the telomerase functioning pathway.

关 键 词：Snapin 人端粒逆转录酶 蛋白相互作用 

分 类 号：Q78[生物学—分子生物学] Q25