抗HIV融合多肽CP32M的制备工艺研究  

Preparation Method of Anti-HIV Fusion Peptide CP32M

作　　者：付超[1] 郭会芹[1] 王孝花[1] 胡洁[1] 戴秋云[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2011年第6期859-863,共5页Letters in Biotechnology

基　　金：新药创制重大专项(2009ZX09103-628);国家自然科学基金(81072676)

摘　　要：目的:研究抗HIV融合多肽CP32M的制备工艺,为其临床前试验奠定基础。方法:采用固-液相混合策略规模合成目标肽,用离子交换色谱、反相高效液相色谱对粗肽进行纯化。结果:获得了利于提高合成及片段缩合效率的3条优选片段,CP32M粗品经DEAE离子交换色谱及C18反相纯化后纯度高于98%。结论:CP32M按3条片段(Ac-1-9-OH、Fmoc-10-21-OH、H-22-32-NH2)划分,可显著提高片段合成及缩合效率,DEAE阴离子交换色谱能除去大部分杂质,提高了第二步C18反相色谱纯化效率。Objective： In order to provide a large quantity of CP32M,an anti-HIV-1 fusion peptide,for its preclinical trial,we probe its large scale of synthesis.Methods： CP32M was synthesized by using combined solid-liquid method,the crude peptide was purified by DEAE anion exchange chromatography and RP-HPLC.Results： Three optimized fragments which were easily synthesized and coupled were found,CP32M with purity more than 98% was obtained after the purification of DEAE anion exchange chromatography and RP-HPLC.Conclusion： The arrangement of three fragments（Ac-1-9-OH,Fmoc-10-21-OH,H-22-32-NH2） of CP32M could significantly elevate the yield of fragment syntheses and couplings,and DEAE anion exchange chromatography could efficiently isolate the impurities and increase the yield of the RP-HPLC purification in the second step.

关 键 词：抗HIV融合多肽 CP32M 多肽合成 固液相混合策略 

分 类 号：Q516[生物学—生物化学] Q503