新疆小麦品种Glu-A3和Glu-B3位点等位变异的分布  

Distribution of Allelic Variations of Glu-A3 and Glu-B3 Loci in Xinjiang Wheat Cultivars

作　　者：聂迎彬[1,2] 穆培源[1,2] 桑伟[1,2] 徐红军[1,2] 庄丽[2,3] 崔凤娟[1,2] 邹波[1] 

机构地区：[1]新疆农垦科学院作物研究所,石河子832000 [2]谷物品质与遗传改良兵团重点实验室,石河子832000 [3]石河子大学生命科学院,石河子832002

出　　处：《麦类作物学报》2011年第5期853-858,共6页Journal of Triticeae Crops

基　　金：兵团科技支疆计划项目(2008ZJ10);兵团博士资金项目(05JC02);国家自然科学基金项目(30560078);兵团科技攻关计划项目(2009GG05);国家科技支撑计划项目(2006BAD01A02-30)

摘　　要：为给新疆小麦品质育种提供理论依据,利用Glu-A3、Glu-B3位点上的17个STS标记检测了185份新疆冬、春小麦品种Glu-A3和Glu-B3位点的等位变异。结果表明,新疆小麦品种以Glu-A3c、Glu-B3a和Glu-B3j亚基为主,其分布频率分别为64.86%、22.70%和17.84%。新疆冬、春小麦品种在Glu-A3位点上均以Glu-A3c亚基为主,分布频率分别为63.30%和67.11%;在Glu-B3位点上,新疆冬、春小麦品种分别以Glu-B3j和Glu-B3a为主,分布频率分别为22.02%和26.32%。新疆冬、春小麦农家品种亚基类型较少,冬小麦农家品种仅有5种类型(以Glu-A3c和Glu-B3i为主),春小麦农家品种有10种类型(以Glu-A3c和Glu-B3d为主)。引进品种和自育品种亚基类型丰富,冬小麦引进品种以Glu-A3c和Glu-B3i为主,分布频率为12.84%和6.42%;春小麦引进品种以Glu-A3c和Glu-B3j为主,分布频率为17.11%和6.58%。冬小麦自育品种以Glu-A3c和Glu-B3j亚基类型为主,分布频率为45.87%和18.35%;春小麦自育品种以Glu-A3c和Glu-B3a亚基类型为主,分布频率为36.84%和18.42%。To clarify the allelic variation of Xinjiang wheat cultivars in Glu-A3 and Glu-B3 loci,and to provide theoretical basis for wheat quality breeding,the allelic variations in Glu-A3 and Glu-B3 alleles were tested among a total of 185 Xinjiang winter and spring cultivars using 17 STS markers.Our results showed： Xinjiang wheat varieties gave priority to with Glu-A3c,Glu-B3a and Glu-B3j,and the distribution frequency was 64.86%,22.70% and 17.84%,respectively.Xinjiang winter and spring cultivars in Glu-A3 alleles were mainly Glu-A3c,with the distribution frequency was 63.30% and 67.11%;meanwhile,in Glu-B3 alleles which were mainly Glu-B3j and Glu-B3a,with the distribution frequency was 22.02% and 26.32%,respectively.Subunits types among landrace cultivars were less than that in Xinjiang wheat cultivars,only 5 types（mainly A3c and B3i） were observed in winter wheat,and 10 types were obserbed in spring wheat（mainly A3c and B3d）.Subunits types were rich in introduced and bred cultivars,introduced winter wheat cultivars were mainly A3c and B3i,and the distribution frequency was 12.84% and 6.42%,respectively;introduced spring wheat cultivars were mainly A3c and B3j,with the distribution frequency was 17.11% and 6.58%,respectively;bred winter wheat cultivars were mainly A3c and B3j,with the distribution frequency was 45.87% and 18.35%,respectively;bred spring wheat cultivars were mainly A3c and B3a,and the distribution frequency was 36.84% and 18.42%,respectively.The distribution frequency of inferior subunits were Glu-A3a,Glu-A3e,and Glu-B3j in Xinjiang wheat cultivars was 5.41%,7.03% and 17.84%,and for high quality subunits Glu-A3d and Glu-B3d is 10.27% and 10.81%,respectively;Which are lower than these of inferior subunits.In addition,17 STS markers were found with good repeatability and stability,and could be quickly and effectively used as support tools in Xinjiang wheat quality improvement.

关 键 词：新疆 小麦 Glu-A3 Glu-B3 等位变异 分子检测 

分 类 号：S512.1[农业科学—作物学] S331