川楝子中香草酸含量测定研究  

Content Determination of Vanillic Acid in Melia toosendan Sieb.

作　　者：杜怡昊[1] 刘建华[2] 李计龙[3] 安立群[3] 杨迺嘉[2] 

机构地区：[1]四川农业大学生命科学与理学院,雅安625014 [2]贵州省生物技术研究开发基地,贵阳550002 [3]贵州大学生命科学学院,贵阳550025

出　　处：《天然产物研究与开发》2011年第6期1103-1106,共4页Natural Product Research and Development

摘　　要：本实验采用薄层色谱法对川楝子中香草酸、异香草酸进行定性鉴别;采用高效液相色谱法对川楝子中香草酸进行含量测定。色谱柱:Hypersil BDS C18(250 mm×4.60 mm,5μm),柱温:30℃,流动相:甲醇∶水∶冰乙酸=25∶75∶0.5,流速:1.0 mL.min-1,检测波长:260 nm。最终,定性鉴别斑点清晰。结果表明香草酸含量在1.022～16.352μg范围内,进样量与峰面积呈良好线性关系,相关系数r=0.9998;香草酸的平均回收率为102.45%,RSD=1.22%(n=6)。In this research, vaninic acid and iso-vanillic acid in Melia toosendan Sieb. were identified by TLC. The content of vanillic acid was determined by HPLC. The HPLC system consisted of BDS-C18 methanol-water-acetic acid （25 ：75：0.5） as mobile phase,flow rate of 1.0 mL/min and column tempreture of 30 ℃. The eluate was detected at 260 rim. The TLC spots developed were fairly clear. The control content of vanillic acid was within the range of 1. 022 - 16. 352 μg, and there was fine linearship between the sample input and the peak area. The average recovery was 102.45%, r=0.9998 （n=6）,RSD = 1.22%.

关 键 词：川楝子 香草酸 异香草酸 薄层色谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]