HPLC法测定不同品种不同产地菊芋叶片中绿原酸的含量  

Determination of Chlorogenic Acid of Different Varieties of Helianthus tuberosus Leaves from Different Areas by HPLC

作　　者：张海娟[1] 黄增荣[1] 隆小华[1] 刘玲[1] 刘兆普[1] 

机构地区：[1]南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室,南京210095

出　　处：《天然产物研究与开发》2011年第6期1107-1109,1159,共4页Natural Product Research and Development

基　　金：国家"863"计划重点资助项目(2007AA091702);公益性行业(农业)科研专项经费项目(200903001-05)

摘　　要：为建立同时测定大丰地区3个菊芋品种及大庆地区2个菊芋品种叶片绿原酸含量的方法,采用高效液相色谱法对不同品种不同产地菊芋叶片中绿原酸含量进行测定。结果表明:1)在检测条件流动相为1%磷酸溶液、乙腈(87∶13)、流速1 mL/min和检测波长327 nm时,绿原酸在0.01～0.1 mg/mL呈良好的线性关系,精密度良好,平均回收率为98.21%,RSD=0.68%;2)大丰地区南芋1号、青芋2号、南芋10号叶片中绿原酸含量分别为干重的0.431%、0.040%和0.933%;3)大庆地区南芋1号、南芋9号叶片中绿原酸含量分别为干重的1.245%、2.139%。研究结果表明菊芋叶片中绿原酸含量在不同品种不同生态区域间有差异,HPLC方法简便、快速、准确,可用于菊芋叶片中绿原酸含量的测定。The aim of this study is to develop a method for determining the content of chlorogenic acid in 3 varieties of Helianthus tuberosus in Dafeng area and 2 varieties of Helianthus tuberosus in Daqing area. Accordingly, HPLC technology was used to determine the chloregenic acid content in the leaves of the Heliartthus tuberosus in different varieties from different areas. The results showed： 1 ） The calibration curve showed a good linear relationship in the range of 0.01-0.1 mg/mL for chlorogenic acid under the conditions of 1% water-phosphoric-ACE （87：13 ） as the mobile phase, a flow rate of 1.0 mL/min and detection wavelength of 327 nm. The precision was fine, and the average recovery rate of chorogenic acid was 98.21%, RSD was 0.68%. 2） In Dafeng area,the chlorogenic acid contents in the leaves of the dry weight of Nanyu No. 1 ,Qingyu No. 2,Nanyu No. 10 were 0. 431% ,0.040% ,0. 933%. 3） In Daqing area,the ehloregenie acid contents in the leaves of the dry weight of Nanyu No. 1 ,Nanyu No. 9 were 1. 245% ,2. 139%. These results suggested that chlorogenic acid content in Helianthus tuberosus leaves was dependent on the variety differences and ecological region differences. This method of HPLC is simple, rapid and accurate and thus is suitable for measuring the ehloregenic acid content in Helianthtts tuberosus leaves.

关 键 词：高效液相色谱 菊芋 绿原酸 品种 产地 

分 类 号：Q949.783.5[生物学—植物学]