口腔白色假丝酵母菌毒力因子与随机扩增多态性DNA条带的多元回归分析  

Establishment of multiple regression model for virulence factors of Saccharomyces albicans by random amplified polymorphic DNA bands

作　　者：刘奇[1] 武有聪[1] 袁有华[1] 白丽[1] 牛坤[2] 

机构地区：[1]大理学院基础医学院医学微生物学及免疫学教研室 [2]大理学院研究生处,大理671000

出　　处：《华西口腔医学杂志》2011年第6期643-645,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30260005);大理学院高层次人才基金资助项目(KY430440);大理学院科研基金青年基金资助项目(KYQN2010-51)

摘　　要：目的探讨白色假丝酵母菌毒力因子与随机扩增多态性DNA(RAPD)电泳条带之间的关系,构建多元回归模型。方法采用体外法对92株白色假丝酵母菌的细胞外磷脂酶活性、分泌性蛋白酶活性、芽管生成率、对口腔黏膜细胞的黏附力进行检测;并通过RAPD方法进行扩增,电泳后分析扩增条带。对上述毒力因子和电泳条带进行多元回归分析。结果白色假丝酵母菌的细胞外磷脂酶活性与RAPD扩增后350、450、650和1300 bp 4个条带明显相关(P<0.05);分泌性蛋白酶活性与350、1 200 bp 2个条带明显相关(P<0.05);芽管生成率与400、550 bp 2个条带明显相关(P<0.05)。结论白色假丝酵母菌RAPD部分电泳条带与部分毒力因子的强弱有关,其所含基因信息可能参与该菌毒力因子的调节。Objective To research the relationship between the virulence factors of Saccharomyces albicans （S.albicans） and the random amplified polymorphic DNA （RAPD） bands of them, and establish the regression model by multiple regression analysis. Methods Extracellu]ar phospholipase, secreted proteinase, ability to generate germ tubes and adhere to oral mucosal cells of 92 strains of S.albicans were measured in vitro; RAPD-polymerase chain reaction（RAPD-PCR） was used to get their bands. Multiple regression for virulence factors of S.albicans and RAPD-PCR bands was established. Results The extracellular phospholipase activity was associated with 4 RAPD bands： 350, 450, 650 and 1 300 bp （P〈0.05）; secreted proteinase activity of S.albicans was associated with 2 bands： 350 and 1 200 bp （P〈0.05）; the ability of germ tube produce was associated with 2 bands： 400 and 550 bp （P〈0.05）. Conclusion Some RAPD bands will reflect the virulence factors of S.albicans indirectly. These bands would contain some important messages for regulation of S.albicans virulence factors.

关 键 词：白色假丝酵母菌 毒力 随机扩增多态性DNA 多元回归 

分 类 号：R379[医药卫生—病原生物学]