高频超声引导注射实现主要组织相容性(抗原)复合物基因大鼠胸腺转移  

Xeno-MHC gene transfer to thymus with high-resolution ultrasound-guided injection

作　　者：许晴[1] 张文谦[2] 李彤[2] 路欣[3] 张进禄[1] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069 [2]首都医科大学附属北京朝阳医院胸外科,北京100069 [3]首都医科大学组织胚胎学教研室,北京100069

出　　处：《中国医学影像技术》2011年第12期2436-2439,共4页Chinese Journal of Medical Imaging Technology

摘　　要：目的探索高频超声引导注射技术实现主要组织相容性(抗原)复合物(MHC)基因大鼠胸腺转移的新方法。方法应用分子生物学技术获得供体Balb/c小鼠异种MHC-I类抗原(H-2Kd)基因,并构建pCI-neo-H-2Kd质粒。对20只成年SD大鼠应用高分辨力超声影像系统获得胸腺标准切面超声声像图,同时使用Vevo轨道系统和超声引导注射针装置,在超声引导下将质粒注入大鼠胸腺内。应用流式细胞仪检测H-2Kd基因在转染大鼠胸腺细胞上的表达。结果超声引导下注射质粒后全部大鼠存活。H-2Kd基因成功转入受体大鼠胸腺,流式细胞仪检测H-2Kd在大鼠胸腺内的表达阳性率为60.73%(56.04%～62.49%)。结论高频超声引导注射技术定位准确,方法简便,对组织损伤轻微,注射成功率较高,可代替手术胸腺内注射法诱导免疫耐受。Objective To explore the feasibility of image-guided intrathymic injection of major histocompatibility complex（MHC） using high-resolution ultrasound.Methods The xenograft MHC-I antigen（H-2Kd） gene was extracted and proliferated from donor Balb/c mice,and the expression vector plasmid of pCI-neo-H-2Kd was constructed with molecular cloning technique.Images on standard section were obtained with high-resolution imaging system.Then the plasmid of pCI-neo-H-2Kd was injected into the thymus of 20 Sprague-Dawley rats with Vevo rail system and ultrasound-guided fine-needle.After injection,expression of H-2Kd in the thymocytes was tested by fluorescence-activated cells sorter（FACS）.Results Injection was successful in all the rats.H-2Kd gene was expressed in the receptors＇ thymus with a transfection efficiency of 60.73%（56.04%—62.49%）.Conclusion Ultrasound-guided intrathymic injection is a safe,effective and convenient way with high degree of target accuracy to perform gene transfection to thymus.This method might induce immune tolerance and instead of open-chest intrathymic injection.

关 键 词：超声检查 胸腺内注射 基因转移 主要组织相容性复合体 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]