土壤农杆菌C_(58)遗传转化东北红豆杉的初步研究  

GENETIC TRANSFORMATION OF TAXUS CUSPIDATA BY AGROBACTERIUM TUMEFACIENS C_(58)

作　　者：盛长忠[1] 王淑芳[1] 张心平[1] 王勇[1] 田俊英[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》1999年第4期27-30,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市自然科学基金!( 9736 0 72 1 1)

摘　　要：用土壤农杆菌C58(AgrobacteriumtumefaciensC58)对东北红豆杉(Taxuscuspidata)不同外植体和不同生长时期的愈伤组织进行了不同时间的感染,结果表明,20d龄的愈伤组织对C58菌比较敏感.感染时间为5min时冠瘿诱导率较高(15.0%).对在无激素B5培养基上得到的白色松软的冠瘿组织分析检测发现,有特异的胭脂碱(nopaline)存在,并具有产生紫杉醇的能力.This paper demonstrates that callus of Taxus cuspidata could be transformed genetically by Agrobacterium tumefaciens C 58 and the bacterial genes transformed into the plant genome could express. Explants and calli of T.cuspidata were inoculated with C 58 for different time and the highest crown gall formation frequency (15.0%) was achieved with 20 day old callus inoculated for 5 minutes. Explants were less susceptibility than calli. In contrast to untransformed callus tissue, the crown gall could proliferate on hormone free medium and produced nopaline. By HPLC analysis, gall cells were able to synthesize taxol (0.0013% dry weight.)

关 键 词：土壤农杆菌C58 东北红豆杉 紫杉醇 遗传转化 

分 类 号：S791.49[农业科学—林木遗传育种] R284.2[农业科学—林学]