^99Tc^m—survivin mRNA反义肽核酸基因显像在肿瘤与炎性反应模型中的对比研究  

Comparative study on imaging of ^99Tc^m-survivin mRNA antisense peptide nucleic acid in tumor and inflammation animal models

作　　者：赵新明[1] 刘亚丽[1] 戴萌[1] 任秀春[1] 王建方[1] 张敬勉[1] 王颖晨[1] 张召奇[1] 赵秀娟[1] 戴春暖[1] 李德志[1] 

机构地区：[1]河北医科大学第四医院核医学科及PET／CT中心,石家庄050011

出　　处：《中华核医学杂志》2011年第6期364-367,共4页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金（81071186）;河北省普通高等学校强势特色学科肿瘤学组项目（冀教高2005[52]）

摘　　要：目的研究^99Tc^m-survivin mRNA反义肽核酸（PNA）显像在肿瘤特异性诊断中的价值。方法用^99Tc^m标记人工合成的12碱基单链survivin mRNA反义PNA。20只荷瘤（A549）裸鼠按随机数字表法分为4组，每组5只。每只裸鼠经尾静脉注入^99Tc^m-survivin mRNA反义PNA，3组进行体内分布研究，其余进行显像研究。用同样方法对20只炎性反应（金黄色葡萄球菌）小鼠进行分组、体内分布和显像研究。采用SPSS13．0软件进行统计学分析，组间计量资料比较采用t检验。结果^99Tc^m-survivin mRNA反义PNA在肝内分布最高，其次是肾，注射后4h肿瘤组靶／非靶（T／NT）比值明显高于炎性反应组，分别为3．69±1．13，2．03±0．47，差异有统计学意义（t=3．01，P=0．02）。肿瘤组在注射药物后0．5h即可见肿瘤清晰显影，至4h时显影仍较清晰，而炎性反应部位始终未见明显显影。结论^99Tc^m-survivin mRNA反义PNA基因显像可通过其在肿瘤组织中的特异性浓聚区分肿瘤与炎性反应，为肿瘤的特异性诊断提供了一种可能的新方法。Objective To investigate the value of ^99Tc^m labeled survivin mRNA antisense peptide nucleic acid （PNA） as an imaging agent in the specific diagnosis for carcinoma. Methods Survivin mRNA antisense PNA was labeled directly with ^99 Tc^m by the ligand-exchange method. Twenty nude mice with lung carcinoma A549 xenografts were randomly divided into 4 groups. Three groups were used for biodistribution study and one group was used for imaging study. Other twenty mice infected by staphylococcus aureus underwent the same procedure. The biodistribution and imaging of ^99Tc^m-survivin mRNA antisense PNA was studied at 1, 2 and 4 h respectively after the intravenous injection in nucle mice bearing lung carcinoma A549 xenografts or inflammation models. SPSS 13.0 was used in the study and all data were analyzed by t test. Results Biodistribution results showed that the highest radioactivity was found in the liver, and then in the kidney. Four hours after the administration of the imaging agent, the radioactivity ratios of target-to- non target （T/NT, tumor or inflammatory lesions to the contralateral regions ） in tumor model group were significantly higher than those in inflammation model group （ 3.69 ± 1.13 vs 2.03 ±0.47, t = 3.01, P = 0. 02 ）. Tumors were clearly visible in the tumor model groups at 0.5 h and still clearly seen at 4 h after the injection of antisense PNA. On the contrary, inflammatory lesions could not be seen clearly. Conclusion ^99Tc^m labeled survivin mRNA antisense PNA can be used to distinguish tumor from inflammation and it may provide a new feasible method for specific tumor diagnosis.

关 键 词：肽核酸 锝 肿瘤细胞 培养的 炎症 放射性核素显像 小鼠 裸 

分 类 号：R817[医药卫生—影像医学与核医学]