阵发性房颤诱发大鼠心房肌电重构特征研究  

Characteristics of atrial electrical remodeling in rats induced by paroxysmal atrial fibrillation

作　　者：汤依群[1] 孙书娟[1] 尹月淼[1] 汪旻晖[1] 黄潞[1] 单娇娇[1] 

机构地区：[1]中国药科大学临床药学教研室

出　　处：《中国临床药理学与治疗学》2011年第11期1205-1209,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家新药创制重大专项(2009ZX09103-088);江苏省自然科学基金(BK2010434)

摘　　要：目的:探讨氯化钙-乙酰胆碱混合液引起的房颤大鼠心房电重构特征。方法:雄性SD大鼠,随机分为正常组和模型组,每组20只。CaCl210mg/mL+ACh 66μg/mL混合液尾静脉注射诱发大鼠房颤,连续诱发7d,正常组静脉注射生理盐水7d。心电图监测大鼠房颤持续时间、心房复极相关心电图参数变化。7d后,观察正常和房颤大鼠心房肌有效不应期,Western-blot法测定心房肌通道蛋白Kv 1.5、Kv 4.2/4.3,钙稳蛋白FKBP 12.6和缝隙连接蛋白Cx40的表达。结果:Ach-CaCl2连续静脉注射引起大鼠房颤持续时间逐渐延长,与正常组相比,房颤大鼠P波变宽,Pd增大,心房有效不应期缩短(P<0.01),同时心房肌Kv 1.5、Kv 4.2/4.3表达下降,FKBP12.6减少,Cx40含量下降(P<0.05)。结论:氯化钙-乙酰胆碱混合液持续给予7d,大鼠心房发生电重构,特征符合或接近临床房颤基质电重构特征。AIM：To investigate the characterisics of atrial electrical remodeling induced by atrial fibrillation（AF） at molecular level.METHODS： Male SD rats were randomized into 2 equal groups.Rats of control group were administrated physiological saline（i.v.） for 7 days.The others were injected ACh-CaCl2（i.v.） for 7 days.AF duration and ECG parameters were measured.On day 8,all rats were anesthetized and the atriums were immediately excised for measuring AERP,the expression of Kv 1.5,Kv4.2/4.3,FKBP12.6 and Cx40 protein were detected using western-blot.RESULTS：In model rats,AF duration continually increased from day 1 to day 7 with ACh-CaCl2 administration.The AERP of model group was shorter than that of control group [（65.3±3.6） ms vs（82.3±0.6） ms;P0.01].Western-blot showed that in the atrium,Kv1.5 and Kv4.2/4.3 expression was down-regulated after 7 days of AF induction,model rats expressed lower levels of Cx40（P0.05,vs control） and FKBP12.6（P0.05,vs control）.CONCLUSION： AF induced by Ach-CaCl2 injection for 7 days,has a stable AF duration,a shortened AERP with atrium ion channel,connexin and calcium regulation protein down-expressed,all of which related closely to the clinical characterics associated with AF.

关 键 词：心房颤动 电重构 缝隙连接蛋白40 

分 类 号：R965.1[医药卫生—药理学]