银叶树品种‘Kelvin Red’启动培养研究  

Study on Initiation Culture of Leucadendron‘Kelvin Red’

作　　者：何丽娜[1] 马琳[1] 姜岩[1] 陈静[1] 潘会堂[1,2] 

机构地区：[1]北京林业大学园林学院,北京100083 [2]国家花卉工程技术研究中心,北京100083

出　　处：《亚热带植物科学》2011年第4期75-78,共4页Subtropical Plant Science

基　　金：国家林业局948项目(2007-4-07)

摘　　要：以银叶树‘Kelvin Red’为试材,对其离体快繁启动培养过程进行了研究。试验结果表明:银叶树外植体最佳的消毒方案为75%酒精浸泡30s,再用0.1%升汞进行5+4 min的二次消毒;最佳取材季节为冬季;最佳外植体类型为茎尖和茎段中上部半木质化部分;最佳培养基配方为WPM+6-BA 1.0 mg/L+IBA 0.5 mg/L。The initiation culture of in vitro rapid propagation in Leucadendron ＇Kelvin Red＇ was investigated.The results showed that the best method for explants disinfection was as follows： the explants were surface-disinfested in a solution of 75% alcohol for 30 seconds and then dipped into 0.1% mercuric chloride two times for 5 minutes and 4 minutes respectively.Winter was the optimal season for collecting explants.It was found that shoot tips and the upper semi-lignified stems were the optimal explants materials.The optimal startup medium for in vitro Leucadendron propagation was WPM ＋ 6-BA 1.0 mg/L ＋ IBA 0.5 mg/L.

关 键 词：银叶树 组织培养 启动培养 

分 类 号：Q943.1[生物学—植物学]