锌缺乏小鼠切牙硬组织发育缺陷的初步研究  

Preliminary study of hard tissue developmental defects in incisors of zinc deficient mice

作　　者：兴海涛[1] 王智明[1] 王川宁[1] 赵志国[1] 张力平[1] 

机构地区：[1]中国医科大学附属盛京医院,口腔科,辽宁沈阳110003

出　　处：《中国美容整形外科杂志》2011年第12期756-758,共3页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 研究锌缺乏致小鼠牙硬组织发育的异常与成釉细胞凋亡的关系.方法 采用HE染色和免疫组化技术,观察纯系CD-1小鼠缺锌后,牙体组织的形态学改变以及成釉细胞中凋亡酶caspase-9的表达情况.结果 实验组（A组）与对照组（B组）相比,小鼠体质量明显偏轻,且毛发枯黄、缺乏光泽,牙体呈淡黄色、磨耗较严重;实验组小鼠牙髓腔内前期牙本质宽于对照组,并出现较多的空泡,细胞数量明显减少,成牙本质细胞排列紊乱,无极性;实验组小鼠成釉细胞中观察到caspase-9呈明显的阳性表达.结论 锌缺乏可以促使成釉细胞中casbase-9表达增高,从而介导小鼠切牙成釉细胞凋亡,并可引起包括牙本质在内的牙硬组织发育缺陷.Objective To explore the relationship of the hard tissue developmental defects in incisors of zinc deficient mice and the apoptosis of ameloblasts. Methods HE staining and immunohistoehemistry were performed to observe the morphological changes of tooth tissue and the expression of caspase-9 of the ameloblasts in the pure CD-I mice with zinc deficiency. Results Compared to the control group, the mouse weight in the experimental group was obviously lower with brown hair, lacking luster, yellowish teeth and much more severe abrasion, the predentin in the dental pulp cavity was wider with more vacuoles, fewer cells, disordered and non-polar odontoblasts. The easpase-9 expression was significantly positive in ameloblasts of the experimental group. Conclusion Zinc deficiency can promote the expression of casbase-9 in the ameloblasts which can mediate apoptosis of ameloblasts and lead to the developmental defects of the hard tissue in the mouse incisors.

关 键 词：锌缺乏 成釉细胞 细胞凋亡 casbase-9 

分 类 号：R622[医药卫生—整形外科] R78[医药卫生—外科学]