解磷黑曲霉L-1菌株原生质体诱变育种  

Protoplast mutagenesis breeding of phosphobacteria Aspergillus niger sp.L-1

作　　者：姬超宏[1] 邓拓[1] 肖敦科[1] 何放[1] 葛绍荣[1] 

机构地区：[1]四川大学生命科学院生物资源与环境教育部重点实验室,四川成都610041

出　　处：《工业微生物》2011年第6期85-88,共4页Industrial Microbiology

摘　　要：以从龙泉山地区酸性红壤中分离的黑曲霉L-1(Aspergillus niger sp.L-1)为出发菌株,通过研究菌株L-1原生质体的形成和再生条件,发现培养30 h的菌丝体在以0.15 mol/L氯化钾为渗透压稳定剂的基本培养基上经过再次培养20 h后,所得菌丝体用0.3%纤维素酶和0.4%蜗牛酶在30℃条件下处理120 min可得大量原生质体,其再生率可达到11.3%。菌株L-1原生质体经过紫外诱变选育后得到L-17,可以使培养基中可溶性磷浓度增加5.7倍,较出发菌株提高3.6倍。L-17在酸性红壤中的解磷能力比解磷巨大芽孢杆菌J1提高2.5倍。Factors affecting protoplast formation and regeneration of the Aspergillus niger sp. L-1 from add red soil of Longquan Mountain were investigated. The protoplast of strain L- 1, cultured for 30 h in a minimal medium containing 0.15 mol/L MgSO4 as oosmotic stabilizer, was processed with 0.3 % cellulase and 0.4 % snail enzyme at 30℃ for 120 minutes. The regeneration frequency of protoplasm could reach up to 11.3 % and cultured for 20 h in a minimal medium again. The protoplast of strain L- 1 was mutated by UV, and then the strain L- 17 was obtained. The results showed that soluble phosphorus concentration in the medium increased 5.7 times, 3.6 times higher than that of the original strain. Ability of phosphorus-dissolving of L- 17 in add red soil increased by 2.5 times than Bacillus. megatherium vat. ]dnosphaticum. J1.

关 键 词：黑曲霉L-1 原生质体诱变 解磷能力 酸性红壤 

分 类 号：Q93[生物学—微生物学]