人脂肪组织NYGGF4基因表达的调控研究  

Study on regulation in expression of NYGGF4 gene in human adipose tissue

作　　者：赵一奇 焦峰 付海龙 赵亚萍 郭锡熔[2] 

机构地区：[1]解放军82医院,江苏淮安223001 [2]南京医科大学附属南京妇幼保健院

出　　处：《西南国防医药》2011年第12期1277-1279,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金(30571978);江苏省医学领军人才项目(LJ200624)

摘　　要：目的探讨肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)对肥胖基因NYGGF4表达的调控作用。方法取适量大网膜脂肪组织进行体外培养,分别以10 ng/ml TNF-α和20 ng/ml IL-6干预6、12、24、48、72 h,应用荧光定量RT-PCR技术检测NYGGF4 mRNA表达水平的变化。结果 (1)TNF-α对人脂肪组织NYGGF4 mRNA表达具有明显的上调作用,且具有明显的时间依赖性,呈现随作用时间延长,效应逐渐增加的趋势。(2)IL-6具有降低人脂肪组织NYGGF4 mRNA表达的作用,但起效较慢,IL-6作用时间与脂肪组织NYGGF4 mRNA水平呈明显负相关关系。结论炎性细胞因子TNF-α、IL-6对人脂肪组织NYGGF4基因的表达呈现不同模式的调控作用,TNF-α可以促进人脂肪组织NYGGF4基因表达,而IL-6对NYGGF4的表达则具有抑制作用。Objective To investigate the regulation effects of tumor necrosis factor-alpha（TNF-α）and interleukin 6（IL-6）on the expression of the obese gene NYGGF4.Methods Proper greater omentum adipose tissues were cultured in vitro and received the intervention of 10 ng/ml TNF-α and 20 ng/ml IL-6 for 6,12,24,48 and 72 h,respectively.The expression level of NYGG4 mRNA was detected by the technology of fluorescent quantitation RT-PCR.Results（1）The expression level of NYGGF4 mRNA was significantly up-regulated by TNF-α in a time-dependent manner.There was a tendency of gradual increase in the effects along with the extension of action time.（2）IL-6 significantly decreased the expression of NYGGF4 mRNA in human adipose tissue,but the effects occurred slowly.There was an obvious negative correlation between the action time of IL-6 and the level of NYGGF4 mRNA in the adipose tissues.Conclusion Inflammatory cytokines TNF-α and IL-6 have different patterns of regulation effects on the expression of NYGGF4 mRNA in the human adipose tissues.TNF-α can promote the expression of NYGGF4 gene,while IL-6 can inhibit the expression.

关 键 词：NYGGF4基因 脂肪组织 肿瘤坏死因子Α 白细胞介素6 

分 类 号：R723.14[医药卫生—儿科]