肾移植慢性排斥反应动物模型的建立与质量控制  

Animal model of renal transplantation chronic rejection:establishment and quality control

作　　者：李健[1] 许亚宏[1] 马小平[1] 谭娟[1] 张艮甫[2] 黄赤兵[2] 陈萍[1] 郭瑜[1] 

机构地区：[1]解放军452医院泌尿外科,成都610021 [2]第三军医大学附属新桥医院泌尿外科

出　　处：《西南国防医药》2011年第12期1297-1299,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的建立一种稳定性好、重复性强的肾移植慢性排斥反应动物模型。方法应用F344-Lewis近交系雄性大鼠作为供受、体构成实验组,供体双肾切取为两个受体供肾;移植肾动、静脉及尿路重建均采用端-端吻合;术中切除受体左侧原肾以植入移植肾,保留受体右侧原肾,术后3 d体外结扎右肾血管。结果完成大鼠同种异体肾移植手术40例,手术时间(90±27)min,取肾时间(25±7)min,植肾时间(37±17)min,供肾热缺血时间(20±5)s,供肾冷缺血时间(37±3)min。20例取肾及40例植肾手术操作均获得成功。获得慢性排斥反应模型35例,移植大鼠存活时间(128±34)d,成功率87.5%。术后10、14 w分别获得移植肾脏轻度及典型慢性排斥反应病理标本。结论正确选择实验动物、熟练显微外科操作技术、减少供肾冷或热缺血时间、合理进行免疫抑制治疗是保障慢性排斥反应动物模型成功建立的关键。Objective To build up a stable and repeatable animal model of renal transplantation chronic rejection.Methods The F344-Lewis I-line male rats were used as donor and recipient.The two kidneys of one donor were respectively transplanted into two recipients.The end-to-end anastomosis was performed in the transplantation of renal artery and vein and the restitution of urin urinary tract.In the operation,the left kidney of the recipient was excised for implantation of the transplanted kidney,and the right kidney was remained.Three days after the operation,the vessels of the right kidney were ligated in vitro.Results Forty cases received the allograft renal transplantation.The operation time was（90±27）min.The time of kidney excision was（25±7）min.The transplantation time was（37±17）min.The warm and the cold ischemia time of transplanted kidneys was（20±5）s and（37±3）min,respectively.The kidney excision in 20 cases and the transplantation in 40 cases were all successful.Thirty five chronic rejection models were obtained.The survival time of the rats receiving transplantation was（128±34）d.The achievement ratio was 87.5%.The pathological specimens of mild and typical renal transplantation chronic rejection were respectively obtained 10 w and 14 w after the operation.Conclusion The key factors of successful establishment of chronic rejection animal model include correct selection of animals,proficiency in microsurgical techniques,reduction of both warm and cold ischemic time and reasonable immunosuppressive therapies.

关 键 词：肾移植 慢性排斥反应 动物模型 质量控制 

分 类 号：R392.4[医药卫生—免疫学]