2％成人AB型血清扩增的骨髓间充质干细胞的分化潜能研究  

Differentiation of Human Bone Marrow Mesenchymal Stem Cells Expanded in 2% AB-adult Serum Medium In vitro

作　　者：胡建立[1,2] 李健 李静[1] 马杰[1] 陈斌[1] 赵春华[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院,北京100005 [2]清华大学第一附属医院,北京100016

出　　处：《中国生物医学工程学报》2011年第6期920-924,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金重点项目(30830052)

摘　　要：寻找一种合适的、可以替代胎牛血清、用以扩增骨髓间充质干细胞的产品,并研究其扩增产品的多向分化潜能。采用贴壁筛选法分离培养成人骨髓间充质干细胞,并利用不同代次的扩增细胞在诱导体系中向成骨细胞、成脂细胞诱导分化。分别采用Vonkossa、碱性磷酸酶染色观察成骨情况,采用油红O染色观察成脂情况。在用2%成人血清配置的完全培养基中,不同代次的成人骨髓间充质干细胞仍旧具有成骨、成脂分化潜能。研究表明,该方法所扩增的成人骨髓间充质干细胞具有成骨、成脂分化潜能,有可能成为理想的体外扩增成人骨髓间充质干细胞的方法。其扩增产品有可能用于骨的组织工程修复。The aim of this study is to find a suitable alternative to fetal bovine serum for amplification of mesenchymal stem cells products and study the differentiation potential of the amplication products. Adherent screening adult bone marrow mesenchymal stem cells were isolated and cultured, and different passages of the expanded ceils were used to differentiate into osteoblasts and fat cells. Osteoblasts were identified by Vonkossa, alkaline phosphatase staining of osteoblasts, and fat cells by oil red staining of adipogenic. In the configuration with 2% adult serum complete medium, different passages of adult bone marrow mesenchymal stem cells still had a bone, adipogenic differentiation potential. The adult bone marrow mesenchymal stem cells expanded in this medium can be incuced into osteoblasts, fat cells. The proposed method may be ideal for expansion of adult bone marrow mesenchymal stem cells in vitro. The amplication products may be useful to bone tissue engineering.

关 键 词：骨髓间充质干细胞 诱导 分化 

分 类 号：R318.08[医药卫生—生物医学工程]