Toll样受体4在多器官功能障碍综合征大鼠肾组织中的表达  

TLR4 expression in the kidney of rats with multiple organ dysfunction syndrome

作　　者：滕林[1] 余旻[2] 李俊明[1] 刘先哲[1] 

机构地区：[1]三峡大学人民医院湖北省宜昌市第一人民医院心血管内科,湖北省宜昌443000 [2]三峡大学人民医院湖北省宜昌市第一人民医院ICU,湖北省宜昌443000

出　　处：《中华急诊医学杂志》2011年第12期1300-1305,共6页Chinese Journal of Emergency Medicine

摘　　要：目的动态监测多器官功能障碍综合征（MODS）大鼠肾组织中Toll样受体4（TLR4）蛋白的表达变化，探讨TLR4在MODS早期肾损伤中的作用。方法实验在三峡大学医学院实验中心完成。将40只成年雄性SD大鼠随机（随机数字法）分为正常对照组（8只）、MODS模型组（32只），模型组又分为造模后6，12，24，48h不同时相，每组8只。采用“二次打击”即眼球失血加腹腔注射脂多糖（LPS）制备MODS模型，对照组予以腹腔注射等量的生理盐水，造模完成后在不同的时相点搜集标本。肉眼、光镜观察肾组织的形态结构变化；采用流式细胞技术（FCM）测定肾组织和外周血白细胞表面TLR4蛋白表达。组间比较采用单因素方差分析（SNK法）。结果（1）正常对照组肾组织结构无改变；MODS组肾组织损伤较重。（2）流式细胞技术结果显示：与对照组比较，造模后6h肾组织TLR4的表达开始增高，但差异无统计学意义，12h达到高峰（P〈0．01），24h开始下降（P〈0．01），48h与对照组差异无统计学意义。与对照组比较，造模后外周血白细胞表面TLR4的表达6～48h组均有明显增加（P〈0．01）。外周血WBC表面的TLR4与肾组织TLR4质量分数变化之间呈正相关，相关系数（r）0．893（P〈0．05）。结论在MODS的早期，大鼠肾组织TLR4表达迅速上调，高表达的TLR4可能在MODS肾损伤的发病中起重要作用。Objective To determine the level of toll-like receptor4 （TLR4） in the kidney of rats with the Multiple Organ Dysfunction Syndrome （MODS）, and to investigate the early phase of kidney damage in MODS. Methods The experiment was done at experimental center of medical college of Three Gorges University. Forty Adult male Sprague-Dawley （SD） rats were randomly （random number） divided into two groups, namely the normal control group and the MODS model group. The rats of model group were further divided into four sub-groups as per different intervals （6 h, 12 h, 24 h and 48 h） , and there were 8 rats in each groups. The animal models of MODS were established by two hits, the left eyeball of each model rat was removed to bleed to 2 mL/100 g, and four hours later, Lipopolysaccharide （ LPS , 5 mg/kg） was injected into intraperitoneal cavity of model rats. The same volume of saline was injected intraperitoneally into rats of control group. All rats were sacrificed at various intervals. The histological changes in the kidney tissue were observed by naked eye and under light microscope. The lever of TLR4 proteins in serum and kidney tissue were detected by using flow cytometry （FCM）. One-way ANOVA was used for comparison among multiple groups. Results （1） There were no histopathological changes in kidney of rats in control group, and the kidney injury was serious in rats with MODS. （2） Compared with the rats of control group, the level of TLR4 in kidney tissue of rats with MODS increased at 6 hours and reached peak 12 hours later （ P 〈 0. 01 ）, and then decreased 24 hours （ P 〈 0. 01 ）. There was no significant difference in level of TLR4 between two groups 6 and 48 hours. Compared with the rats of control group, the level of TLR4 in peripheral blood leucocyte of rats with MODS increased significantly 6 - 48 hours after LPS （ P 〈 0.01 ）. The concentrations of serum and kidney tissue TLR4 proteins were positively correlated with each other （ r = 0. 893, P 〈 0. 05）. Con

关 键 词：多器官功能障碍综合征 TOLL样受体4 脂多糖 全身炎症反应综合征 肾组织 病理改变 二次打击 大鼠 

分 类 号：R285.5[医药卫生—中药学]