突变型沼泽红假单胞菌的构建及颜色变化的评价  

Constructing blue-green mutants and evaluating its color change for Rhodopseudomonas palustris

作　　者：戴静[1] 李建忠[1] 全亚玲[1] 郝葆青[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《西南民族大学学报（自然科学版）》2011年第6期908-912,共5页Journal of Southwest Minzu University(Natural Science Edition)

摘　　要：基于野生沼泽红假单胞菌与其突变菌株间的颜色变化,探索和研制全细胞传感器具有重要的现实意义.实验通过除去沼泽红假单胞菌中蓝绿基因crtI,构建其绿色突变菌株,比较了菌体间颜色变化;同时检测了突变株积聚无色的类胡萝卜素前体——番茄红素的能力.试验表明,突变体菌株在510—570nm波长范围内其吸收光谱作用明显降低,说明呈显绿色的主要原因是由于细菌叶绿素IIa的存在,以及光合膜中胡萝卜素组成成分的改变.采用CIE-L*a*b*色彩空间坐标评价绿色突变体菌株与野生型菌株的颜色变化,计算出绿色突变型和野生型菌株之间的色彩差异(△E*ab)值.实验数据表明基于类胡萝卜素菌株研制的全细胞传感器能够对菌体颜色变化(绿色到红色)更为敏感和更易区分.It is of great significance that the whole-cell biosensors are explored and studied on the color change between the green mutants and their wild-type strains for Rhodopseudomonas palustris.In this study,the blue-green mutant is constructed by deleting the crtl gene from the wild-type of Rhodopseudomonas palustris;the change of color between the green mutants and their wild-type strains is compared,and meanwhile,the ability of accumulating the color-less carotenoid precursor is tested.The result shows that the absorption of the blue-green mutants is decreased observably at 510-570 wavelength range.It indicates that the greenish bacterial colors are mainly caused by the existence of bacterichlorophy II and the changes in carotenoid composition of the photosynthetic membrane.The colors of the blue-green mutants and their wild-type strains are plotted in the CIE-L＊a＊b＊ color space,and the color difference（ΔE＊ab） values between a green mutant and its wild type are calculated.The data show that it is more susceptive and distinguishable to the change in bacterial color from green to red from a reporter signal of carotenoid-based wholecell biosensors.

关 键 词：沼泽红假单胞菌 类胡萝卜素 突变菌株 蓝绿基因 细胞传感器 

分 类 号：Q81[生物学—生物工程]