不同水平蚕沙对新西兰兔盲肠微生物区系的影响  

Effect of different levels of silkworm feces on caecum microbial community of New Zealand rabbit

作　　者：艾丹[1,2] 郭春华[1,2] 严锦绣[1,2] 张艳[1,2] 余淼[1,2] 谢晓红[3] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]动物遗传育种学国家民委-教育部重点实验室,成都610041 [3]四川省畜牧科学研究院

出　　处：《西南民族大学学报（自然科学版）》2011年第6期913-917,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：西南民族大学研究生"创新性科研项目"(CX201161);国家兔产业技术体系专项经费资助(CARS-44-B-4)

摘　　要：研究饲料中添加蚕沙对新西兰兔盲肠微生物区系的影响.选取5组共15只新西兰兔,1组对照组,4组试验组,每组3只.对照组饲喂基础日粮,试验组(试验Ⅰ、Ⅱ、Ⅲ和Ⅳ组)分别饲喂添加6%、12%、18%、24%蚕沙的基础日粮,无菌采集盲肠内容物.盲肠细菌16SrDNA的V3可变区经PCR扩增,扩增产物经DGGE电泳后再进行相似性分析.结果表明,饲喂蚕沙后,各试验组盲肠细菌DGGE图谱的条带数与对照组差异不显著(P>0.05),各组间DGGE图谱的相似性小于30%.蚕沙添加水平影响新西兰兔盲肠细菌区系.This paper investigates the effect of silkworm feces on the caecum microflora of meat New Zealand rabbits using 16S rDNA-based approaches.Fifteen rabbits are allocated in 5 groups of 3 each.The control group is fed on basal concentrate,while the treatment groups（treatmentⅠ,Ⅱ,Ⅲ and Ⅳ group） are fed on diets added 6%,12%,18% and 24% silkworm feces,respectively.The cecal contents are collected and the regions V3 of the16 SrDNA of cecal bacteria are amplified.DGGE profiles of the PCR amplicons are compared by similarity analysis.Tabbits of the treatment groups show a higher bacterial diversity compared with those of the control group.The silkworm feces show apparent effect on the DGGE profiles.

关 键 词：蚕沙 肉兔 PCR/DGGE 盲肠微生物区系 

分 类 号：S816.7[农业科学—饲料科学] S829.1[农业科学—畜牧学]