加拿大原产地西洋参多糖提取工艺优化研究  

The optimization of water extraction technology of polysaccharide from Panax quinquefolium L.collected from original place in Ontario of Canada by single factor and orthogonal designed method

作　　者：任烨[1] 李厚聪[2] 刘永恒[1] 黄艳菲[1] 李艳丹[1] 丁玲[1] 彭镰心[3] 刘圆[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041 [2]湖北民族学院医学院,湖北恩施445000 [3]成都大学生物产业学院,四川成都610106

出　　处：《西南民族大学学报（自然科学版）》2011年第6期940-945,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家外专局外国文教专家重点项目(2010);四川省杰出青年学术技术带头人后续计划(2011JQ0051)

摘　　要：[目的]筛选加拿大原产地西洋参多糖的最佳提取工艺条件。[方法]单因素和正交试验法。[结果]加拿大原产地西洋参多糖的最佳提取分离工艺为:加热回流法,药材粒径为65目的西洋参粉末加入25倍体积80%乙醇90℃回流1 h,提取2次,滤渣减压干燥后按照料液比为1:35加水浸泡3 h,100℃提取1 h,提取3次。[结论]为加拿大原产地西洋参多糖的提取分离条件提供了参考,为加拿大原产地多糖西洋参的进一步开发研究提供依据。Objective：To screen the water extraction technology of total polysaccharide from Panax quinquefolium L.collected from original place in Ontario of Canada.Methods：Single factor and designed L9（34） orthogonal are adopted.Result： The best water extraction technology is as follows： Recirculation,65 particle sieve size of powders,added 25 times of with ethanol concentration 80 % for 1 h and two times,extraction temperature 90 ℃.The residues adds water after vacuum dries,material to solvent 1：35,3 h immersed time,100 ℃ extraction temperature for per water extraction,and total extraction time is 3.Conclusion：This experiment establishes the fundament of further research on Panax quinquefolium L.collected from original place in Ontario of Canada.

关 键 词：西洋参 加拿大原产地 多糖 提取工艺 正交设计 

分 类 号：R284.2[医药卫生—中药学] R93[医药卫生—中医学]