吸烟对慢性阻塞性肺疾病患者稳定期支气管肺泡灌洗液中T细胞功能影响的临床研究  

Effect of smoking on T lymphocytes in bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease during their stable periods

作　　者：陈菁[1] 徐清[1] 杨硕[1] 刘梦琳[1] 聂红[1] 孙洁民[1] 

机构地区：[1]武汉市第一医院呼吸科,430022

出　　处：《国际呼吸杂志》2011年第23期1780-1782,共3页International Journal of Respiration

基　　金：基金项目：湖北省自然科学基金资助项目（2005ABA210）

摘　　要：目的评价吸烟对慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）患者稳定期支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中T细胞功能的影响。方法流式细胞术测定吸烟的COPD患者稳定期BALF中CD3＋、CD4＋、CD8＋T细胞和CD4＋／CD8＋水平。结果与非COPD组相比，COPD患者稳定期BALF中CD3＋、CD4＋、CD4＋/CD8＋水平明显下降（P〈0．05），CD8＋明显升高（P〈0．05）；在COPD患者中，稳定期BALF中CD8＋、CD4＋、CD8＋T细胞无明显差异（P〉0．05），但持续吸烟组CD4＋／CD8＋明显下降（P〈0．05）。结论COPD患者稳定期气道T细胞功能明显异常，其中持续吸烟的COPD患者气道CD4＋／CD8＋下降最为显著。Objective To investigate the effect on T lymphocytes in bronchoalveolar lavage fluid （BALF） influenced by smoking in patients with chronic obstructive pulmonary disease （COPD） during their stable periods. Methods To test the levels of CD3＋ ,CD4＋ ,CD8＋ ,CD4＋ /CD8 ＋ in BALF in patients enrolled by flow cytometry. Results The levels of CD3＋ ,CD4＋ ,CD4＋/CD8＋ in BALF in patients with COPD during their stable periods decrease compare with normal group （ P 〈0.05） while the level of CD8＋ increases （ P 〈0.05）. There is no difference among the levels of CD3＋ ,CD44 , CD8＋ in patients with COPD （ P 〉 0.05）, yet the level of CD4＋/CD8＋ decreases apparently in patients who smoke continuedly （ P 〈0.05）. Conclusions Continued smoking leads to the lower T lymphocytes＇ function in patients＇ airways with COPD during their sable periods.

关 键 词：吸烟 慢性阻塞性肺疾病 支气管肺泡灌洗液 T细胞 

分 类 号：R563.9[医药卫生—呼吸系统]