慢性HBV感染者肝组织内HBcAg、HLA-DrAg的表达及意义  

Expression and Significance of HBcAg and HLA DrAg in Hepatic Tissues of Patients with Chronic HBV infected

作　　者：王利华[1] 赵洪涛[1] 李淑香 

机构地区：[1]山东省立医院,250021 [2]菏泽地区人民医院

出　　处：《山东医药》1999年第23期5-7,共3页Shandong Medical Journal

摘　　要：应用免疫组化技术，对１１６ 例慢性乙型肝炎病毒（ＨＢＶ）感染者肝组织中的乙肝病毒核心抗原（ＨＢｃＡｇ）、人类白细胞相关抗原（ＨＬＡ－ＤｒＡｇ）进行了检测。结果显示，ＨＢｃＡｇ 在慢性ＨＢＶ感染者肝组织中的总阳性率为５０％ ，慢性活动性肝炎（ＣＡＨ）患者肝组织中浆膜型ＨＢｃＡｇ 的表达明显高于慢性迁延性肝炎（ＣＰＨ）患者及肝组织无明显异常者（ＡＳＣ），而核型ＨＢｃＡｇ 的表达在ＣＡＨ、ＣＰＨ及ＡＳＣ组没有显著差异；浆膜型ＨＢｃＡｇ 的表达随肝组织炎症活动指标（ＨＡＩ）评分值的增高而增强，而核型ＨＢｃＡｇ 的表达强度与ＨＡＩ评分值的高低无关。光镜下发现，浆膜型ＨＢｃＡｇ 多分布于碎屑样坏死及灶性坏死区，且浆膜型ＨＢｃＡｇ 阳性肝细胞常有各种变化，或明显萎缩，或肿胀变形。ＨＬＡ－ＤｒＡｇ 在慢性ＨＢＶ感染者肝组织中总阳性率为２１．６％ ，其表达强度与肝脏炎症指标无相关关系，但光镜下ＨＬＡ－ＤｒＡｇ阳性肝细胞多分布于坏死灶周围，且ＨＬＡ－ＤｒＡｇ 阳性肝细胞变性坏死明显。浆膜型ＨＢｃＡｇ 与ＨＬＡ－ＤｒＡｇ 两者同时阳性的慢性ＨＢＶ感染者较单纯浆膜型或核型ＨＢｃＡｇ 阳性或ＨＬＡ－ＤｒＡｇ 阳性者炎症损伤重，且其ＨＡＩ评分值较高，?The expression of HBcAg and human leueocyte histo compatibility antigen(HLA DrAg)in hepatic tissues of 116 patients with chronic HBV infected were studied by the immunohistochemical method.The results showed that total positive rates of HBcAg were 50%.Positive rates of hepatocytic cytoplasm membrane staining of HBcAg in chronic active hepatitis (CAH) were more higher than in ASC and chronic persistent hepatitis (CPH).Whereas nuclear staining were no significant difference among ASC,CPH and CAH.There was positive relationship between hepatocytic cytoplasm membrane staining of HBcAg and hepatic inflammatory degree.There was no relationship between nuclear staining and hepatic inflammatory degree.Hepatocytes of cytoplasm membrane staining of HBcAg were always arround necrotic sites and associated with obvious degeneration and necrosis.HLA DrAg was low expression.There was no relationship between its expression and hepatic inflammatory degree.But positive hepatocytes of HLA DrAg were associated with obvious degeneration and necrosis.Chronic hepatitis B with simutaneously positive staining of cytoplasm membrane staining of HBcAg and HLA DrAg has more serious inflammatory injury than with cytoplasm membrane staining or nuclear staining of HBcAg or HLA DrAg.The results indicate that HBcAg and HLA DrAg are associated with immune lesion in chronic hepatitis B.They maybe have coordinated action.

关 键 词：乙型肝炎 HBV HBCAG HLA-DrAg 

分 类 号：R512.620.2[医药卫生—内科学]