APA微囊牛肾上腺嗜铬细胞治疗疼痛的量效时效关系研究  

Study on Relationship of Dose-effect and Time-effect of APA Microencapsulated Bovine Chromaffin Cells on Pain Treatment

作　　者：回建峰[1] 李涛[2] 杜智[2] 宋继昌[2] 

机构地区：[1]天津市第四中心医院,天津300140 [2]天津市第三中心医院,天津市人工细胞重点实验室,天津300170

出　　处：《生物医学工程学杂志》2011年第6期1145-1147,1153,共4页Journal of Biomedical Engineering

基　　金：国家863项目资助(102-09-05-07);天津市科委攻关项目资助(003113611);天津市科委重点攻关项目资助(013105911)

摘　　要：研究海藻酸钠-聚赖氨酸-海藻酸钠(APA)微囊牛肾上腺嗜铬细胞(BCC)治疗大鼠疼痛动物模型的量效时效关系。用结扎坐骨神经制作大鼠疼痛动物模型,将APA微囊BCC移植到动物模型的蛛网膜下腔,分别用冷致痛实验和热过敏实验观察镇痛效果。结果显示与对照组相比,细胞数量5万组、10万组和20万组收缩差异均减小(P<0.05),且随细胞量增加差异减小,表明有明显镇痛作用;40万组与20万组相比没有明显差异。这种镇痛作用可持续12周以上。以上结果表明APA微囊BCC治疗大鼠疼痛动物模型的移植细胞数量和镇痛效果呈正相关关系,有效镇痛时间在12周以上。This study was to investigate the relationship of dose-effect and time-effect of Alginate-Polylysine-Alginate （APA） microencapsulated bovine chromaffin cells on the treatment of pain model rats. Using a rat model of painful peripheral neuropathy, the antinociceptive effects of APA microencapsulated bovine cells transplanted into the subarachnoid space was evaluated by cold allodynia test and hot hyperalgesia test. Compared with control group, the withdrawal difference with cell number 50 thousands groups, 100 thousands groups and 200 thousands groups was reduced （P〈0. 05）, and the difference decreased with the cells increases, indicating a significant analgesic effect. There was no significant difference between 400 thousands groups and 200 thousands groups. This analgesic effect maintained longer than 12 weeks. There was a positive correlation between the analgesic effect and the quantity of APA mieroencapsulated bovine chromaffin cells which were transplanted to treat pain model rats, and the effective antinoeiception remained longer than 12 weeks.

关 键 词：APA微囊牛肾上腺嗜铬细胞 坐骨神经结扎 冷致痛实验 热过敏实验 

分 类 号：R402[医药卫生—临床医学]