聚醚醚酮-羟基磷灰石-碳纤维复合材料的体外细胞相容性研究  

Research on the Extracorporeal Cytocompatibility of a Composite of HA,Carbon Fiber and Polyetheretherket-one

作　　者：刘学勇[1] 邓纯博[2] 刘吉泉[1] 李建军[1] 隋国鑫[3] 

机构地区：[1]中国医科大学附属盛京医院骨科,沈阳110004 [2]沈阳医学院奉天医院骨科,沈阳110024 [3]中国科学院金属研究所,沈阳110006

出　　处：《生物医学工程学杂志》2011年第6期1159-1164,共6页Journal of Biomedical Engineering

基　　金：沈阳市科委基金资助项目(F-10-205-1-48)

摘　　要：将大鼠成骨细胞与聚醚醚酮-羟基磷灰石-碳纤维(PEEK-HA-CF)复合材料间接和直接培养,通过CCK-8对复合材料的细胞毒性进行评价;流式细胞仪测定材料对细胞增殖指数的影响;测定复合培养后成骨细胞的碱性磷酸酶活性变化;应用扫描电镜(SEM)观察成骨细胞形态学变化。结果表明PEEK-HA-CF复合材料无细胞毒性,复合材料组胞增殖指数与空白对照组比无差异,3d时高于钛合金组。第7d时,PEEK-HA-CF组的碱性磷酸酶活性升高。SEM下,细胞在复合材料上生长良好。PEEK-HA-CF复合材料具有良好的细胞相容性,有希望成为一种新的骨科植入材料。The present research was to study the biocompatibility of a composite of hydroxyapatite （HA）, carbon fiber （CF） and polyetheretherket-one （PEEK） by co-culturing with the osteoblasts in vitro. Cell relative growth （RGR） was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8. The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites. Morphological properties of the osteoblast cells in vitro were observed by scanning electro-microscopy （SEM）. The PEEK-HA- CF materials have no cytotoxicity to osteoblasts. The proliferation index of PEEK-HA-CF was higher than that of Ti alloy group, but these was no significant difference compared to that of control group. The ALP activity was the highest on PEEK-HA-CF composites surface after 7 days. The osteoblast cells co-cultured with the PEEK-HA-CF composite were adhered welt to the biomaterial as observed under the SEM. The results suggested that the PEEK- HA-CF composites had good biocompatibility in vitro and might be a novel orthopedic implanted material.

关 键 词：聚醚醚酮 羟基磷灰石 碳纤维 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]