体外培养猕猴穆勒细胞经诱导可表现出视网膜前体细胞特征  

Rhesus monkey (Macaca mulatta) muller cells exhibit retinal stem/progenitor cell features in vitro

作　　者：郭祥玉[1] 金立方[1] 纪少珲[1] 魏强[1] 牛昱宇[1] 季维智[1] 

机构地区：[1]中国科学院昆明动物研究所灵长类研究中心云南省动物生殖生物学重点实验室,云南昆明650223

出　　处：《Zoological Research》2011年第6期611-616,共6页动物学研究（英文）

基　　金：科技部重大专项(2009ZX09501-028);云南社会科技发展计划(2007GH);中科院知识创新工程重大项目(KSCX1-YW-22)

摘　　要：近年来的研究发现,鸡、大鼠和人视网膜中的穆勒胶质细胞(muller cell)在一定条件下,可显示出视网膜干/前体细胞的功能。但是,有关人类近亲猕猴穆勒细胞的研究尚未见报道。该研究首先使用基础培养基(DMEM+10%FBS)建立猕猴的穆勒细胞系,其表达GS、vimentin、CRALBP和EGFR等穆勒细胞标记,不表达GFAP,与人的穆勒细胞基因表达相似而与啮齿类差异较大。穆勒细胞经神经干细胞培养基培养一周后可表达pax6、nestin、sox2、otx2、six6和six3等视网膜干细胞标记,继续使用视黄酸诱导,部分细胞可分化为神经元细胞,这说明猕猴的穆勒细胞在体外诱导条件下,可去分化为视网膜前体细胞,有望成为视网膜疾病细胞替代疗法的一种细胞来源。Recent evidences indicate that retinal muller ceils exhibit retinal progenitor characteristics under certain condition in chick, rat and human. However, there is no report on nonhuman primate, a close relative to human. In this study, we first established a muller cell line of rhesus monkey expressing GS, vimentin, CRALBP, EGFR, barely GFAP, which resemble the expression profile of human muller cells, differ from that of rodent. Expression of pax6, nestin, sox2, otx2, six6, and six3 was detected after one week culture in neural stem cell medium. Further culture with retinoic acid induced some cells differentiate toward neuron. These results suggest that primate muller cell is capable of dedifferentiating to retinal progenitors, which may serve as a potential celt source for cell therapy to treat retinal degenerative diseases.

关 键 词：猕猴 视网膜干细胞 穆勒细胞 视网膜疾病 

分 类 号：Q813.11[生物学—生物工程] Q959.848