PTEN在小鼠卵细胞和早期胚胎发育中的功能研究  

Role of PTEN in mouse pre-implantation development

作　　者：王喜宏[1,2] 和协超[1] 韩树标[1] 季维智[1] 郑萍[1] 

机构地区：[1]中国科学院昆明动物研究所,云南昆明650223 [2]中国科学院研究生院,北京100049

出　　处：《Zoological Research》2011年第6期647-650,共4页动物学研究（英文）

摘　　要：PI3K/AKT信号通路在哺乳动物早期胚胎发育中起重要作用。抑癌基因PTEN是该通路中的负调节因子,但PTEN在卵和早期胚胎中的表达、分布以及作用都还未见报道。本研究通过免疫荧光方法发现卵细胞及着床前胚胎都表达PTEN,且具活性的PTEN主要分布在生发泡期(germinal vesical,GV)卵细胞的皮层部位以及致密桑椹胚的卵裂球表面。在培养基中添加低浓度的PTEN特异性抑制剂bpV(pic),GV期卵母细胞的成熟不受影响,但着床前胚胎发育受到阻滞。该结果提示PTEN在小鼠着床前胚胎发育中可能起重要作用。The PI3K/Akt signal transduction pathway plays an important role in pre-implantation embryonic development. The tumor suppressor gene PTEN negatively regulates the PI3K/Akt pathway. Although PI3K is constitutively activated during pre-implantation embryonic development, currently no evidence shows the presence and possible involvement of PTEN in early embryo development. We investigated the expression of PTEN protein in germinal vesicle （GV） stage oocytes as well as in pre-implantation embryos. The activated form of PTEN was distributed in the peripheral of GV oocytes and compact morula. Treatment of GV oocytes with PTEN inhibitor bpV（pic） did not affect the maturation of the oocyte, but significantly impaired embryonic development. Thus, our study suggests the necessary role of PTEN in pre-implantation embryonic development.

关 键 词：卵细胞 着床前胚胎发育 PTEN BPV 

分 类 号：Q954.4[生物学—动物学] Q593.4