脑微血管内皮细胞缺氧模型转化生长因子β1表达与脑溢安的干预  

Effects of Naoyian serum on transforming growth beta 1 mRNA expression in cultured rat cerebral microvascular endotheal cells with hypoxia

作　　者：万赛英[1] 黎杏群[2] 智屹惠[2] 罗云[2] 

机构地区：[1]广州中医药大学附属佛山中医院,广东省佛山市528000 [2]中南大学湘雅医院中西医结合研究所,湖南省长沙市410008

出　　处：《中国组织工程研究与临床康复》2011年第46期8625-8629,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家中医药管理局基金资助项目(2000-J-B-03)~~

摘　　要：背景:临床及动物实验证实脑溢安能促进脑出血急性期血肿吸收及神经功能恢复,提高生活质量。目的:观察脑溢安血清对体外缺氧培养的大鼠脑微血管内皮细胞转化生长因子β1mRNA表达的影响。方法:用分离培养的大鼠脑微血管内皮细胞移入厌氧培养箱培养18h建立缺氧损伤模型,并随机分为正常组、模型组、正常血清组及含脑溢安血清组。正常组为同一批正常培养的脑微血管内皮细胞;模型组将正常培养的细胞放入厌氧培养箱内培养18h;正常血清对照组细胞在培养液中加5%正常血清后,放入厌氧培养箱中培养18h;脑溢安血清组细胞在培养液中加5%脑溢安血清后,放入厌氧培养箱内培养18h。结果与结论:缺氧培养使脑微血管内皮细胞存活数量减少,其表达的转化生长因子β1mRNA增强,而脑溢安血清能明显增加脑微血管内皮细胞存活数量,降低转化生长因子β1mRNA表达水平(P<0.05)。说明脑溢安血清下调转化生长因子β1mRNA的表达可能是其抑制脑微血管内皮细胞的凋亡对缺氧损伤脑微血管内皮细胞的保护作用机制之一。BACKGROUND： Clinical and animal experiments have confirmed that Naoyian （NYA） serum can promote hematoma absorption during acute cerebral hemorrhage and subsequent neurological function recovery and improve patients＇ living quality. OBJECTIVE： To determine the effects of NYA serum on the expression of transforming growth β1 （TGF-β1） mRNA in cultured rat cerebral microvascular endothelial cells （RCMECs） with hypoxia. METHODS： RCMECs were cultured with the culture medium consisting of 5% NYA serum or 5%normal serum in an anaerobic incubator for 18 hours so as to establish the hypoxia models. The cultured RCMECs were randomly divided into four groups： normal, model, normal serum and NYA serum. The viability of RCMECs was determined by MTT method. TGF-β1 mRNA expression was measured by reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS AND CONCLUSION： The cultured RCMECs exhibited damage after 18 hours of hypoxia injury. The number of surviving RCMECs in NYA serum group was significantly higher than that in the model and normal serum groups, as determined by MTT method. TGF-β1mRNA expression was increased by hypoxia in the cerebral microvascular endothelial cells. TGF-β1mRNA expression in the NYA serum group was significantly lower than that in the other groups （P 〈 0.05）. These findings suggest that NYA serum downregulating TGF-β1mRNA expression may be one of mechanisms by which NYA serum inhibits apoptosis of cerebral microvascular endothelial cells and then protects cerebral microvascular endothelial cells from hypoxia injury.

关 键 词：转化生长因子Β1 脑微血管内皮细胞 缺氧损伤 中药脑溢安 血清药理 大鼠 

分 类 号：R318[医药卫生—生物医学工程]