整合素α_5β_1在成骨细胞与生物衍生材料黏附过程中的表达  

Expression of integrin alpha 5 beta 1 during the adhesion of osteoblasts to bio-derived materials

作　　者：祁洁[1] 张姝江[2] 刘宗智[1] 

机构地区：[1]陕西省人民医院骨科,陕西省西安市710068 [2]广州医学院第一附属医院骨科实验室,广东省广州市510120

出　　处：《中国组织工程研究与临床康复》2011年第47期8762-8764,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：陕西省科学技术研究发展计划项目(2008K14-07);陕西省自然科学基础研究计划项目(2009JM4017)~~

摘　　要：背景:整合素在细胞与材料的黏附过程中发挥重要作用。目的:了解整合素α5β1在成骨细胞构建组织工程骨和组织工程骨膜过程中的表达,探讨在成骨细胞与生物衍生材料黏附过程中整合素α5β1发挥的作用。方法:选用人胚骨膜来源成骨细胞为种子细胞,接种生物衍生骨及羊膜支架材料上,制备组织工程骨及骨膜,分别培养2,4,6,8,10d。单纯培养的成骨细胞作为对照组。结果与结论:实时定量PCR测定结果,培养早期整合素α5表达呈阴性,整合素β1组织工程骨中的表达略高于组织工程骨膜,培养2,6d组比较差异有显著性意义。单纯细胞培养的对照组中,整合素β1稳定,各时间段比较差异无显著性意义。提示生物衍生材料有利于成骨细胞黏附,但在黏附过程的初期,成骨细胞可能是通过纤维连接蛋白以外的其他蛋白质完成该过程的。BACKGROUND： Integrin plays an important role in the cell adhesion process. OBJECTIVE： To explore the expressions and function of integrinα5β1 during the adhesion of osteoblasts to bio-derived materials. METHODS： Fetal osteoblasts as seed cells were used to construct tissue engineered bone and periosteum following incubation on the bio-derived materials, which were cultured for 2, 4, 6, 8, 10 days in vitro. Osteoblasts cultured alone served as controls. RESULTS AND CONCLUSION： Integrinα5 did not express at the early-stage. Integrinβ1 kept expressing in all groups, and expressed higher on bio-derived bone than on tissue-engineered periosteum, especially at the 2nd and 6th days. Integrinβ1 expressed stably in the control group. The bio-derived materials promote osteoblasts adhesion but fibronectin is not essential to the adhesion of osteoblasts.

关 键 词：生物衍生材料 成骨细胞 整合素Α5Β1 黏附 种子细胞 

分 类 号：R318[医药卫生—生物医学工程]