多孔丝素复合脂肪间充质干细胞支架修复兔尿道缺损能促进血管的形成  

Role of adipose-derived mesenchynal stem cell combined with silk fibroin scaffold in enhancing vascular formation in rabbit urethral repair

作　　者：张亚[1] 周云[1] 贾立山[1] 翟景梅[1] 苗小芬 王睿[2] 

机构地区：[1]苏州大学附属儿童医院外科,江苏省苏州市215003 [2]苏州中医院病理科,江苏省苏州市215009

出　　处：《中国组织工程研究与临床康复》2011年第47期8777-8781,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江苏省卫生厅面上科研课题项目(H200819)"细胞复合型丝素支架修复尿道缺损的应用前期研究";苏州市社会发展科技项目(SS08021)"应用脂肪间充质干细胞复合多孔丝素支架修复尿道缺损"~~

摘　　要：背景:应用脂肪间充质干细胞复合丝素支架修复尿道缺损,国内外尚无公开报道。目的:观察脂肪间充质干细胞复合多孔丝素支架在修复兔尿道缺损中促进血管形成的作用。方法:取兔附睾处脂肪垫组织,分离培养脂肪间充质干细胞。将大白兔按随机数字表法设尿道缺损对照组、多孔丝素膜修复组、脂肪间充质干细胞复合多孔丝素膜修复组。结果与结论:第3代脂肪间充质干细胞CD44、Vimentin均呈阳性表达。造模后2,4,6周苏木精-伊红染色结果显示,脂肪间充质干细胞复合多孔丝素膜组和多孔丝素膜组较尿道缺损对照组、脂肪间充质干细胞复合多孔丝素膜组较多孔丝素膜组血管形成多。造模后4周,应用FⅧ-RAg免疫组织化学染色法计数微血管密度,脂肪间充质干细胞复合多孔丝素膜组和多孔丝素膜组高于尿道缺损对照组(P<0.05),脂肪间充质干细胞复合多孔丝素膜组高于多孔丝素膜组(P<0.05)。提示脂肪间充质干细胞型丝素支架在尿道缺损的修复中能显著促进血管的形成、生长。BACKGROUND： We searched the relevant research papers on main domestic and foreign online retrieval databases in recent five years, and no publication was found on the uretral defect repair with adipose-derived mesenchymal stem cells （ADMSCs） on silk fibroin scaffold. OBJECTIVE： To study the effect of ADMSCs combined with a porous silk fibroin scaffold （PSFS） on enhancing vascular formation in rabbit urethral repair. METHODS： The ADMSCs were separated from the epididymal fat pads of the rabbits, and then treated with cell isolation and culture. In animal study, the rabbits were assigned into group A with the urethral defects, group B with urethral defects repaired by PSFS, and group C with urethral defects repaired by ADMSCs seeded on PSFS. RESULTS AND CONCLUSION： The expression of CD44 and Vimentin was positive on ADMSCs of third passage by indirect immunoflurescence methods. On histological examination, blood vessel formation in group C was significantly more abundant than groups A and B in the 2nd to 6th week after operation. The FⅧ-RAg positive microvessel density was also significantly more than groups A and B in the 4th week after operation （P 0.05）. ADMSCs combined with silk fibroin scaffold can promote in vivo vascularization for urethral repair.

关 键 词：尿道缺损 脂肪间充质干细胞 多孔丝素 支架 血管 

分 类 号：R318[医药卫生—生物医学工程]