壳聚糖/聚乳酸羟基乙酸组织工程化神经植入犬体内的慢性生物相容性  

Chronic biocompatibility of chitosan/poly(lactic-co-glycolic acid) tissue engineered nerve transplanted into Beagles

作　　者：朱奇[1,2] 杨宇民[2] 吴坚[2] 胡文[2] 顾晓松[2] 

机构地区：[1]南通大学公共卫生学院预防医学教研室,江苏省南通市226019 [2]南通大学江苏省神经再生重点实验室,江苏省南通市226001

出　　处：《中国组织工程研究与临床康复》2011年第47期8842-8846,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家863重大项目(2006AA02A128)~~

摘　　要：背景:2个月以内短期生物相容性实验显示,壳聚糖、聚乳酸和聚羟基乙酸对大鼠外周神经均无毒性,可作为组织工程化神经材料。目的:评价壳聚糖/聚乳酸羟基乙酸组织工程化神经植入Beagle犬体内6个月后的慢性生物相容性。方法:在壳聚糖神经导管中插入聚乳酸羟基乙酸纤维制备成组织工程化神经,移植桥接Beagle犬坐骨神经50mm缺损,同时以Beagle犬50mm自体神经移植作为对照组。结果与结论:植入壳聚糖/聚乳酸羟基乙酸组织工程化神经6个月后,Beagle犬精神、食欲、活动等一般情况良好,体质量增加与对照组相当;植入后2,4,6个月血液学和血清生化检测结果与对照组无明显差异;再生神经及其周边组织未出现变性、坏死,心、肝、脾、肺、肾等主要脏器大体解剖和组织切片未见异常。表明壳聚糖/聚乳酸羟基乙酸组织工程化神经植入Beagle犬体内6个月后慢性生物相容性良好。BACKGROUND： Short-term biocompatibility test within 2 months has showed that chitosan, polylactic acid and polyglycolic acid have no toxicity in rat peripheral nerve, which can be used as tissue engineered nerve materials. OBJECTIVE： To evaluate the chronic biocompatibility of the chitosan/poly（lactic-co-glycolic acid） （PLGA） tissue engineered nerve transplanted into beagles. METHODS： The tissue engineered nerve was prepared through inserting the PLGA filaments into chitosan conduits, and then transplanted into beagles bridging sciatic nerve 50 mm defect, and autologous nerve graft was taken as control at the same time. RESULTS AND CONCLUSION： The status, appetite and activity of beagles which were transplanted with chitosan/PLGA tissue engineered nerve were good and the weight gain were similar to the autologous nerve graft group. Hematology and serum biochemical tests results after 2, 4, 6 months were also similar to the control group and there were no significant difference between two groups. Necrosis and degeneration was not found in the regenerated nerve as well as the surrounding tissues. And the tissue biopsies of the heart, liver, spleen, lung, kidney and other major organs were not abnormal. The chronic biocompatibility of the chitosan/PLGA tissue engineered nerve which was transplanted into Beagles for 6 months was good.

关 键 词：壳聚糖 聚乳酸羟基乙酸 组织工程化神经 慢性生物相容性 Beagle犬 

分 类 号：R318[医药卫生—生物医学工程]