中国HIV感染者的IL-21水平及其在高效抗反转录病毒治疗中的动态变化(英文)  

IL-21 level in Chinese HIV infected individuals and its dynamics undergoing HAART

作　　者：郑力文[1] 加路[2] 陈霞[2] 郑煜煌[2] 何艳[2] 周华英[2] 谌资[2] 罗艳[2] 

机构地区：[1]中南大学湘雅医学院,长沙410078 [2]中南大学湘雅二医院感染科,艾滋病研究室,长沙410011

出　　处：《中南大学学报（医学版）》2011年第11期1059-1064,共6页Journal of Central South University ：Medical Science

基　　金：supported by National Science and Technology Important Item of China(2008ZX10001-008,20008ZX10005-003,2009ZX10005-015)

摘　　要：目的:观察在接受抗HIV治疗的中国HIV感染者队列中血白细胞介素21(IL-21)的动态变化。方法:将符合高效抗反转录病毒治疗(HAART)指南标准的25例慢性HIV成年感染者纳入研究,在启动HAART的0,6,12个月时各抽取感染者的20 mL血液。运用流式细胞仪进行CD4+T细胞和CD8+T细胞计数,以RT-PCR检测HIV的RNA水平,ELISA法测定IL-21的水平。结果:中国HIV感染者的IL-21水平低于正常人,在接受HAART治疗过程中逐渐升高,但未达到正常人水平。IL-21的水平和CD4+T细胞数呈正相关,但与CD8+T细胞数无关;HIV RNA的水平与CD4+T细胞数呈负相关,但与CD8+T细胞数无关。结论:IL-21与HIV免疫致病机制有一定关系,并在抗反转录病毒治疗的免疫重建中起重要作用。Objective To investigate the dynamics of interleukin-21（IL-21） cytokine in the Chinese HIV patients undergoing highly active antiretroviral therapy（HAAPT）.Methods A total of 25 adults with chronic HIV infections,responding to combined highly active antiretroviral therapy（HAART） guideline criteria were enrolled for a 1-year follow-up.After signing an informed consent,20 mL blood was collected from each patient at the base line,6 month and 12 month,respectively.CD4 and CD8 cell count was quantified by flux cytometry,serum HIV RNA quantified by real time PCR and IL-21 concentrations by ELISA.Results IL-21 levels increased gradually during the follow-up but did not reach the healthy levels.IL-21 correlated positively with the CD4 cells but not with CD8 T cells.HIV RNA correlated negatively with CD4 cell count but did not show any relationship with the CD8 cells.Conclusion IL-21 has potential role in the immunopathogenesis of HIV,and might be an important factor in immune construction during HAART.

关 键 词：人类免疫缺陷病毒 高效抗反转录病毒治疗 白介素21 CD4细胞 CD8细胞 HIV RNA 

分 类 号：R512.91[医药卫生—内科学]