镇静小鼠的非侵入式气道反应性测定法及药物作用  

A new non invasive method for the measurement of airway responsiveness in sedated mice and effects of drugs

作　　者：陆智勇[1] 张纬萍[1] 吕可真 蒋欣欣 朱朝阳 康桦 魏尔清 

机构地区：[1]浙江大学湖滨校区药理教研室,杭州310031

出　　处：《中国现代应用药学》1999年第6期8-11,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金!(No.39770865) 资助

摘　　要：目的：建立并验证镇静小鼠气道反应性的非侵入式测定法，探讨小鼠气道高反应性与气道炎症的关系。方法：观察致敏及药物对小鼠引喘阈浓度的影响，及支气管肺泡灌洗液（ＢＡＬＦ） 中白细胞渗出量的变化。结果：与未致敏小鼠相比，致敏小鼠吸入ＯＡ６ｈ 的ＭＣｈ 引喘阈浓度显著降低，ＢＡＬＦ 中白细胞渗出量显著增高，地塞米松（７ ．５ｍｇｋｇ） 和氨茶碱（３７ ．５ ｍｇｋｇ） 可降低致敏小鼠吸入ＯＡ 引起的气道反应性增高和ＢＡＬＦ 中白细胞渗出的增加。结论：本模型可有效地检测小鼠气道反应性，抗原引起的小鼠气道高反应性与气道炎症有关。OBJECTIVE:To establish and verify a non invasive method for quantitative measurement of airway responsiveness(AR) in diazepam sedated mice,and to investigate the relationship between airway hyperresponsiveness and airway inflammation in mice.METHOD:The effects of sensitization and drugs on the wheezing threshold concentration to methacholine(MCh),and the changes of the number of leukocytes in bronchoalveolar lavage fluid(BALF) in mice were observed.RESULTS:Lower wheezing threshold concentration to methacholine and more leukocytes in BALF were found in ovalbumin(OA) sensitized mice than those in non sensitized mice repectively after repeated antigen inhalation(0.5%,20 min per day,for 6 days).Dexamethasone(7.5mg/kg,ip.bid,for 3 days) and aminophylline(37.5mg/kg,ip.bid,for 3 days) inhibited these effects induced by antigen.CONCLUSION:This mouse method is useful to detect the changes in AR of mice,and there was a relationship between the airway hyperresponsiveness and airway inflammation induced by antigen in the sensitized mice.

关 键 词：气道反应性 小鼠 哮喘 抗原 地塞米松 

分 类 号：R562.250.5[医药卫生—呼吸系统]