经前期综合征肝气郁证大鼠海马雌激素受体α和β研究  

Study on Estrogen Receptors α and β in the Hippocampus of Premenstrual Syndrome Model Rats of Gan-qi Depression Syndrome

作　　者：高杰[1] 宋春红[2] 徐凯勇[2] 郭英慧[1] 张惠云[2] 乔明琦[1] 

机构地区：[1]山东中医药大学基础医学院,济南250355 [2]山东中医药大学中医药经典理论教育部重点实验室,济南250355

出　　处：《中国中西医结合杂志》2011年第12期1645-1650,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2011CB505102);国家自然科学基金资助项目(No.30930110;30973688;81072721);中国博士后基金资助项目(No.20090451343);山东省博士后基金资助项目(No.200803033)

摘　　要：目的研究经前期综合征(premenstrual syndrome,PMS)肝气郁证大鼠海马雌激素受体(estrogenreceptor,ER)α和β分布模式、蛋白表达和功能活性的改变情况以及调肝方药经前舒颗粒的相应作用靶点,为深入探索PMS肝气郁证病机和经前舒颗粒的作用机制提供线索。方法将SD大鼠随机分为正常组、模型组和给药组,每组7只。模型组和给药组采用居住入侵法进行造模。给药组大鼠造模同时给予经前舒颗粒10 mL/kg灌胃;模型组和正常组大鼠给予同等体积灭菌水灌胃,均每天1次,连续5天。运用免疫组织化学法、Western blot和葡聚糖-活性炭单点测定法分别检测各组大鼠海马中ERα和ERβ定位、蛋白水平及与雌激素的结合活性。结果各组大鼠海马中ERα和ERβ分布无明显差异;海马整体及CA1、CA3区内ERα和ERβ蛋白水平以及与雌激素结合活性方面,模型组较正常组均明显升高(P<0.05),给药组较模型组则明显下降(P<0.05)。结论海马高雌激素水平及ERα、ERβ表达和活性增强可能是PMS肝气郁证的重要病机和经前舒颗粒的作用靶点。Objective To study the distribution pattern,the protein expressions,and changes of functional activities of estrogen receptor（ER） α and β in the hippocampus of premenstrual syndrome（PMS） rats of Gan-qi depression syndrome（GDS）,and to find out corresponding effect targets of Jingqianshu Granule（JG）,thus providing clues for exploring the pathogenesis of PMS of GDS and the mechanisms of JG.Methods SD rats were randomly divided into three groups,i.e.,the normal group,the model group,and the medication group,7 in each.Resident intruder stress was used to establish the model in the model group and the medication group.JG was given to rats in the medication group at the dose of 10 mL/kg by gastrogavage while modeling.Equal volume of sterilized water was given to rats in the model group and the normal group,once daily,for 5 successive days.Then the location,protein levels,and ligand-binding capacities of ERα and ERβ in the hippocampus of rats in three groups were detected using immunohistochemical assay,Western blot,and dextran-active carbon binding assay.Results There was no difference in the distribution pattern of ERα and ERβ in the hippocampus of the three groups.In aspects of protein levels and estrogen-binding capacities of ERα and ERβ in the hippocampus,CA1 and CA3 regions,they increased more obviously in the model group than in the normal group（P0.05）,while they decreased more significantly in the medication group than in the model group（P0.05）.ConclusionHigher estrogen levels and enhanced expressions and activities of ERα and ERβ in the hippocampus might be important mechanisms for PMS of GDS,which might also be the effect targets for JG.

关 键 词：经前期综合征 肝气郁证 雌激素受体 海马 

分 类 号：R271.115[医药卫生—中医妇科学]