3T3-L1脂肪细胞与胰岛素抵抗脂肪细胞miRNAs表达谱的差异性分析  

Differential expression profiles of miRNAs between 3T3-L1 adipocytes and insulin-resistant adipocytes

作　　者：凌宏艳[1] 胡弼[1] 庹勤慧[2] 刘刚[2] 奉水东[3] 朱炳阳[2] 廖端芳 

机构地区：[1]南华大学医学院生理学教研室,湖南衡阳421001 [2]南华大学药物药理研究所药理学教研室,湖南衡阳421001 [3]南华大学公共卫生学院流行病教研室,湖南衡阳421001 [4]湖南中医药科大学药学院,湖南长沙410208

出　　处：《中国现代医学杂志》2011年第31期3856-3860,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30971170和No:81000328);南华大学博士科研启动基金(No:2010XQD39)

摘　　要：目的探讨正常3T3-L1脂肪细胞和胰岛素抵抗(IR)脂肪细胞微小RNA(miRNAs)的差异性表达,并进行初步分析。方法采用高糖(25 mmol/L葡萄糖)/高胰岛素液(1μmol/L)处理3T3-L1脂肪细胞24h,通过葡萄糖摄取实验判断IR脂肪细胞模型的建立;接着通过miRNA芯片技术,检测脂肪细胞和IR脂肪细胞中miRNAs的表达,并对其分析;随后借助生物信息学方法,对显著变化的2个miRNAs(miR-320和miR-21)寻找并筛检其可能调控的靶基因。结果共获得79个IR相关miRNAs(29个低表达,50个高表达,其中miR-320显著上调,miR-21显著下调),且筛选出与IR或糖尿病相关的靶基因共13个。结论获得了3T3-L1脂肪细胞和IR脂肪细胞miRNAs的差异性表达谱,为进一步研究这些miRNAs在IR和糖尿病的发病机制中的作用奠定了基础。【Objective】 To investigate differential expression profiles of miRNAs between normal 3T3-L1 adipocytes and insulin-resistant adipocytes（IR-adipocytes）,and analyze their functions preliminarily.【Methods】 IR-adipocyte model was induced with high glucose（25 mmol/L） and high insulin（1μmol/L） in combination（high glucose/ insulin） for 24 hours.To identify the success of IR-adipocyte model,-glucose uptake was detected.Then the profile alteration of miRNAs was examined and analyzed by miRNA microarray analysis.Subsequently,the potential target genes of the miR-320 and miR-21 were predicted and screened by means of Bioinformatics.【Results】 According to a microarray-based screening,79 insulin resistance related miRNAs were obtained,including 50 highly-expressed miRNAs and 29 lowly-expressed ones.Among these miRNAs,miR-320 was markedly up-regulated,while miR-21 down-regulated;and 13 potential insulin-resistant or diabetes-related target genes of the two miRNAs were predicted by informatics analysis.【Conclusions】 MiRNA expression profiles in 3T3-L1 adipocytes and IR-adipocytes have been obtained,so as to lay necessary basis for further research on the function of miRNAs in IR and diabetes.

关 键 词：miRNAs表达谱 3T3-L1脂肪细胞 胰岛素抵抗 差异性分析 

分 类 号：R587.102[医药卫生—内分泌] R-332[医药卫生—内科学]