对虾白斑综合症病毒(WSSV)囊膜表面蛋白的研究  

Study on the Envelop Surface Protein of White Spot Syndrome Virus (WSSV)

作　　者：秦崇涛[1,2,3] 胡向明[4] 杨丰[3] 潘大仁[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建中医药大学中西医结合学院,福州350108 [3]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005 [4]厦门大学生命科学学院,福建厦门361005

出　　处：《中国农学通报》2011年第29期72-75,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金(No.31072243);公益性行业(农业)科研专项(No.201103034)

摘　　要：利用生物素标记转移技术对牛血清白蛋白(BSA)和凡纳滨对虾的鳃细胞膜蛋白进行标记,研究其与对虾白斑综合症病毒(WSSV)之间的相互作用。发现分子量在50～56kD之间的两种蛋白质位于WSSV的表面,并且与鳃细胞膜蛋白具有特异性的相互作用。根据分子量推测可能是病毒的两种膜蛋白,VP52A和VP56。In this study,using the biotin label transfer technique,we found that the biotin label was transferred from BSA or gill member protein of Litopenaeus vannamei to the envelop protein of WSSV.Western blotting showed that two proteins whose molecular weight among 50-55 kD were labeled with biotin.It was concluded that the two proteins was location on the surface of the WSSV viron and could interact with the gill member protein of Litopenaeus vannamei.They were estimated to be VP52A and VP56.

关 键 词：对虾白斑综合症病毒 生物素标记转移 囊膜表面蛋白 

分 类 号：Q78[生物学—分子生物学]