黄檗茎段不定芽分化过程中生理生化指标变化研究  

Studies on Changes of Physiology and Biochemistry Indexes During Adventitious Shoot Regeneration from Stem Explants of Phellodendron amurense

作　　者：王慧梅[1] 于海娣[1] 陈亚杰[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《中国农学通报》2011年第28期36-39,共4页Chinese Agricultural Science Bulletin

基　　金：哈尔滨市科技创新人才研究专项资金项目(2010RFLXN017);中央高校基本科研业务费专项资金项目(DL10CA01);黑龙江省博士后科研启动金项目(LBH-Q07011)

摘　　要：以黄檗茎段为外植体,研究其不定芽分化过程中生理生化指标的变化。将黄檗无菌苗的茎段接种到MS+1.5mg/LBA+0.5mg/LNAA+20g/L葡萄糖的培养基上,于20天左右大量形成不定芽,30天左右形成小苗。通过比色法,对茎段不定芽再生过程的生理生化指标测定表明,在不定芽形成过程中,SOD酶活性呈现出逐渐下降的趋势,而其他2种抗氧化酶(CAT和POD)活性、可溶性蛋白及可溶性糖含量在不定芽形成的高峰期之前均呈不同程度的上升趋势,随后则呈下降趋势。试验表明,抗氧化酶活性,可溶性蛋白及可溶性糖含量与不定芽再生有着明显的相关性。The stem segments were used as explants to study the changes of physiology and biochemistry indexes during adventitious shoot regeneration.The stem segments from aseptic seedling of Phellodendron amurense were inoculated on MS medium containing 1.5 mg/L 6-BA,0.5 mg/L NAA and 20 g/L glucose.After about 20 days of culture adventitious shoots were induced,and occurred to regenerated plantlets at 30 days or so.By using colorimetry,the SOD activity showed downtrend during the formation of adventitious shoots.While the other antioxidant enzyme（CAT and POD） activities,the concent of soluble protein as well as soluble sugar all increased in varying degrees before peak of adventitious shoot formation,and then decreased.The results showed that there was obvious correlation between antioxidant enzyme activities,the concent of soluble protein and soluble sugar and adventitious shoot regeneration.

关 键 词：不定芽再生 可溶性蛋白 可溶性糖 抗氧化酶 

分 类 号：Q945[生物学—植物学]