亚洲百合试管苗生根的研究  

Research on Rooting of Asian Lily Plantlets

作　　者：刘婕[1] 刘雅莉[1] 贾敏[1] 王岩[1] 

机构地区：[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学林学院,陕西杨凌712100

出　　处：《中国农学通报》2011年第28期163-166,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"百合ACC合成酶反义基因构建及其遗传转化"(30371013)

摘　　要：为了优化亚洲百合生根培养基配方,为亚洲百合转基因植株试管苗的生根移栽奠定一定的基础,以亚洲百合'普利安娜'(Lilium Asiatic hybrid cv.'Pollyanna')和'普瑞头'(Lilium Asiatic hybrid cv.'Prato')试管苗为材料,探讨5种不同激素的浓度组合对试管苗生根率、生根数量、最长根长的影响。结果表明:不同植物激素的组合和浓度对亚洲百合的生根有显著影响,且2个品种生根状况有一定的差异。在生根培养基为MS+2,4-D1.0mg/L+KT0.1mg/L+6-BA1.0mg/L+蔗糖30g/L+琼脂7g/L时,亚洲百合'普利安娜'平均生根率达到98.73%,生根数量达到6.53条,最长根长为4cm;生根培养基为:MS+NAA1.0mg/L+TDZ0.1mg/L+蔗糖30g/L+琼脂7g/L时,亚洲百合'普瑞头'平均生根率达到92.73%,生根数量达到6.63条,最长根长为3.77cm。最后得出:亚洲百合'普利安娜'最适宜的生根培养基为:MS+2,4-D1.0mg/L+KT0.1mg/L+6-BA1.0mg/L+蔗糖30g/L+琼脂7g/L;亚洲百合'普瑞头'的适宜生根培养基为:MS+NAA1.0mg/L+TDZ0.1mg/L+蔗糖30g/L+琼脂7g/L。In order to optimize the formulation of Asian lily rooting medium and lay a foundation for rooting and transplanting of Asian lily transgenic plants,Lilium Asiatic hybrid cv.＇ Pollyanna ＇ and Lilium Asiatic hybrid cv.＇ Prato ＇ test-tube seedlings were used to study the effect of combinations of five different hormone in different concentrations on rooting rate,root number,longest root length.The results showed that：a combination of different types and concentrations of plant hormones significantly affected Asiatic lily rooting,and the rooting status of the two species had some differences.When using the rooting medium：MS＋2,4-D 1.0 mg/L＋KT 0.1 mg/L＋6-BA 1.0 mg/L＋sucrose 30 g/L＋ agar 7 g/L,the average rooting rate of Lilium Asiatic hybrid cv.＇ Pollyanna ＇ could reach 98.73%,the root number of them could reach 6.53 cm and the longest root length of them was 4 cm.When using the rooting medium：MS＋NAA 1.0 mg/L＋TDZ 0.1 mg/L＋sucrose 30 g/L＋ agar 7 g/L,the average rooting rate of Lilium Asiatic hybrid cv.＇ Prato ＇ could reach 92.73%,the root number of them could reach 6.63 cm and the longest root length of them was 3.77 cm.The most appropriate rooting medium of Lilium cv.＇ Pollyanna ＇ was：MS＋2,4-D 1.0 mg/L＋KT 0.1 mg/L＋6-BA 1.0 mg/L＋sucrose 30 g/L＋ agar 7 g/L;the most suitable rooting medium for Lilium cv.＇ Prato ＇ was：MS＋NAA 1.0 mg/L＋TDZ 0.1 mg/L＋ sucrose 30 g/L＋agar 7 g/L.

关 键 词：亚洲百合 植物激素 生根 

分 类 号：S682.29[农业科学—观赏园艺]