层黏连蛋白和纤维连接蛋白在小鼠肾小体发育中的表达  

Expression of laminin and fibronectin in mouse of renal corpuscle development and maturation

作　　者：张萍[1] 田鹤[1] 郭敏[1] 

机构地区：[1]辽宁医学院组织学与胚胎学教研室,辽宁锦州121001

出　　处：《中国现代医学杂志》2011年第32期3988-3991,共4页China Journal of Modern Medicine

基　　金：辽宁省教育厅资助项目(No:L2010274)

摘　　要：目的观察层黏连蛋白(LN)和纤维连接蛋白(Fn)在小鼠肾小体发育过程中的表达规律,探讨其与肾小体发生发育的关系及作用。方法采用免疫组织化学技术结合体视学方法定量检测LN和Fn的表达。结果胚龄12 d的胎鼠LN表达于输尿管芽周围,胚龄14 d始,在各期肾小体的基膜均有表达。体视学测量发现,随着肾小体的发育成熟,其阳性表达的面密度值逐渐增多。Fn在除逗号小体以外的各期肾小体的基底膜处均有表达。体视学测量结果显示其表达量逐渐增加。结论 LN和Fn在小鼠肾小体发育和成熟过程中发挥重要作用。[Objective] To observe the chronological and spatial expressions of laminin （LN） and fibronectin （Fn） in developing mouse renal corpuscle and investigate the relationship between the expression and renal corpuscle development. [Methods] Immunohistochemistry and stereological were used to observe and measure the expression of LN and Fn in embryos and postnatal mouse renal corpuscle, [Results] The expression of laminin was noticed in the ureteric bud, all stages of glomeruli and increased gradually with ages; The expression of Fn was also noticed in all stages of giomeruli except for the comma-shaped corpuscles, and also increased gradually with ages. [Conclusion] LN and Fn play an important role in the development and maturation of mouse renal corpuscle.

关 键 词：肾小体 发育 层黏连蛋白 纤维连接蛋白 免疫组织化学 体视学 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]