钙拮抗剂尼莫地平对映体在Chiralcel OJ柱上的拆分机理  

Separation mechanism of calcium antagonists nimodipine enantiomers on Chiralcel OJ column

作　　者：张燕[1] 唐晓丹[2] 王绍艳[2] 丛景香[2] 田雨[1] 

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051 [2]辽宁科技大学分离技术中心,辽宁鞍山114051

出　　处：《化学试剂》2011年第12期1117-1120,共4页Chemical Reagents

基　　金：辽宁省教育厅创新团队项目(2008T093);辽宁科技大学大学生科研训练项目第六期(2010006)

摘　　要：采用高效液相色谱法(HPLC),在Chiralcel OJ手性柱上拆分尼莫地平。以正己烷-异丙醇为流动相,研究了异丙醇体积含量、流速及柱温对尼莫地平对映体拆分行为的影响。并结合溶质计量置换保留模型和热力学理论对尼莫地平的手性识别机理进行探讨。实验表明V(正己烷)∶V(异丙醇)=90∶10,流速0.6 mL/min,柱温25℃时,尼莫地平对映体可实现良好分离。在实验的异丙醇浓度范围内,前一个对映体lgI值和Z值均小于后一个对映体。热力学函数焓变和熵变之差为负值,分离对映体满足||ΔΔH0||>||ΔΔS0||,手性拆分过程为焓控过程。Nimodipine was directly resolved by high performance liquid chromatography on the Chiralcel OJ chiral column the influences of the volume of isopropanol,flow rate and temperature on the separation of nimodipine were investigated.The chiral recognition mechanism of Chiralcel OJ chiral column was studied by stoichiometric displacement model for retention and thermodynamic theory.The results showed that good separation of nimodipine was available in the mobile phase of hexane/isopropanol（90∶10,V/V） with a flow rate of 0.6 mL/min and the column temperature of 25 ℃.In the experimental rage of concentration of isopropanol,the lgI values and Z values of the former enantiomer of nimodipine was higher than that of the later enantiomer.The enthalpy change difference and entropy change difference were negative values,meanwhile the enantioseparation meeting ｜｜ΔΔH0｜｜｜｜ΔΔS0｜｜ to indicate that the process of chiral sepatation was an enthalpy-controlled process.

关 键 词：高效液相色谱(HPLC) CHIRALCEL OJ手性柱 尼莫地平 计量置换保留模型 热力学 

分 类 号：R917[医药卫生—药物分析学]